Qin Yingfei, Wu Xiyang, Tan Xinqiu, Liu Yong, Zhang Deyong, Chen Yue
LongPing Branch, College of Biology, Hunan University, Changsha 410125, China.
State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
J Agric Food Chem. 2025 Jul 30;73(30):18804-18821. doi: 10.1021/acs.jafc.5c07599. Epub 2025 Jul 17.
The fungus (. ) triggers rice blast, significantly endangering the agricultural production. We identified a potential antifungal metabolite, Dibutyl Phthalate (DBP), from the fermentation broth of WXY53 through mass spectrometry analysis. DBP inhibited the growth of hyphae by 44.52%, inhibited the formation of appressorium with an inhibition rate of 85.07% at 8 h and 68.97% at 24 h, and directly delaying the colonization of invasive hyphae on plants. Treatment with DBP substantially decreased the lesion count by 57.61% and 43.77% in rice and barley, respectively. The metabolomics and transcriptome sequencing analyses showed that DBP affects lipid metabolism, the MAPK signaling pathway, carbohydrate metabolism, and other metabolic processes of . Significant differences were found in the levels of reactive oxygen species, enzymatic activity, lipid metabolism, conductivity, extracellular protein content and membrane fluidity after DBP treatment. Scanning electron microscopy revealed that the hyphae and conidia fractured, wrinkled, and deformed. DBP does not significantly affect plant seedlings growth and has an LC50 of 11.93 mg/L for . And DBP may have broad-spectrum antifungal activity. In this study, we provided a reference for the ability of DBP to inhibit and revealed its antifungal mechanism.
该真菌（.）引发稻瘟病，严重危及农业生产。我们通过质谱分析从WXY53的发酵液中鉴定出一种潜在的抗真菌代谢产物邻苯二甲酸二丁酯（DBP）。DBP抑制菌丝生长44.52%，在8小时时抑制附着胞形成的抑制率为85.07%，在24小时时为68.97%，并直接延缓侵入菌丝在植物上的定殖。用DBP处理分别使水稻和大麦的病斑数大幅减少57.61%和43.77%。代谢组学和转录组测序分析表明，DBP影响该真菌的脂质代谢、丝裂原活化蛋白激酶（MAPK）信号通路、碳水化合物代谢及其他代谢过程。DBP处理后，在活性氧水平、酶活性、脂质代谢、电导率、细胞外蛋白质含量和膜流动性方面发现了显著差异。扫描电子显微镜显示菌丝和分生孢子断裂、起皱并变形。DBP对植物幼苗生长无显著影响，对该真菌的半数致死浓度（LC50）为11.93 mg/L。并且DBP可能具有广谱抗真菌活性。在本研究中，我们为DBP抑制该真菌的能力提供了参考，并揭示了其抗真菌机制。
