Devkar Heena U, Juyal Kartik, Thakur Narsinh L, Kaur Parvinder, Parmar Keyur, Pullapanthula Radhakrishnanand, Narayanan Shridhar
CSIR- National Institute of Oceanography, Dona Paula, 403004, Goa, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Curr Microbiol. 2025 May 6;82(6):280. doi: 10.1007/s00284-025-04262-6.
Antimicrobial resistance (AMR) is one of the leading health crises worldwide that demands new antimicrobials to enter the clinical pipeline. Marine sponges are a rich source of promising bioactive compounds. Due to their sessile nature and filter-feeding lifestyle, sponges are prone to attack by competitors, predators, and pathogens. To combat these threats, they produce a diverse array of bioactive compounds. Notably, the microbial communities residing within the sponges make many of these beneficial compounds. Twenty-one bacterial isolates from various marine sponges from the Indian coast were selected for this study. The bacterial isolates were fermented to obtain crude extracts, which were then screened against critical bacterial pathogens. Based on the MIC (minimum inhibitory concentration) results, two isolates, Bacillus velezensis NIO_002 and Stutzerimonas stutzeri NIO_003 showing good activity, were characterized by morphological, biochemical, and molecular methods. Genome mining predicted multiple antibiotic biosynthetic gene clusters, most of which showed a high degree of similarity to known gene clusters, and some with low or no similarity which may be indicative of novel gene clusters. LC-MS (liquid chromatography-mass spectrometry) data revealed the putative presence of certain antibacterial compounds previously reported in the literature. To our knowledge, this is the first study to report the antimicrobial activity of marine sponge-associated Bacillus velezensis and Stutzerimonas stutzeri strains characterized by whole genome sequencing, thereby indicating the novelty of our strains. This study emphasizes the potential of our bacterial isolates for further development as a source of promising antibiotics to address the escalating challenge of drug-resistant pathogens.
抗菌耐药性（AMR）是全球主要的健康危机之一，需要有新的抗菌药物进入临床研发流程。海洋海绵是有前景的生物活性化合物的丰富来源。由于其固着的特性和滤食性的生活方式，海绵容易受到竞争者、捕食者和病原体的攻击。为了应对这些威胁，它们产生了各种各样的生物活性化合物。值得注意的是，生活在海绵体内的微生物群落产生了许多这些有益的化合物。本研究从印度海岸的各种海洋海绵中挑选了21株细菌分离株。对这些细菌分离株进行发酵以获得粗提物，然后针对关键细菌病原体进行筛选。根据最低抑菌浓度（MIC）结果，通过形态学、生化和分子方法对两株表现出良好活性的分离株——贝莱斯芽孢杆菌NIO_002和施氏假单胞菌NIO_003进行了鉴定。基因组挖掘预测了多个抗生素生物合成基因簇，其中大多数与已知基因簇具有高度相似性，还有一些与已知基因簇相似度低或无相似性，这可能表明存在新的基因簇。液相色谱 - 质谱联用（LC - MS）数据揭示了文献中先前报道的某些抗菌化合物的假定存在。据我们所知，这是第一项报道通过全基因组测序鉴定的海洋海绵相关贝莱斯芽孢杆菌和施氏假单胞菌菌株抗菌活性的研究，从而表明了我们分离菌株的新颖性。本研究强调了我们的细菌分离株作为有前景的抗生素来源进一步开发的潜力，以应对耐药病原体不断升级的挑战。
