Matos Julyana Sthéfanie Simões, Demoliner Meriane, Gularte Juliana Schons, Filippi Micheli, de Abreu Góes Pereira Vyctoria Malayhka, da Silva Mariana Soares, Weber Matheus Nunes, de Barros Marcelo Pereira, Spilki Fernando Rosado
Environmental Quality Graduate Program, Molecular Microbiology Laboratory, Feevale University, Novo Hamburgo 93525-075, Brazil.
Virology Master Program, Molecular Microbiology Laboratory, Feevale University, Novo Hamburgo 93525-075, Brazil.
Pathogens. 2025 Mar 24;14(4):310. doi: 10.3390/pathogens14040310.
The surveillance of wildlife viromes is essential for identifying zoonotic threats within the One Health framework. This study analyzed rectal and oral swabs from 88 individuals representing 13 species as felids, wild rodents, marsupials and non-human primates in Southern Brazil using metagenomic sequencing. ( = 15 individuals) and ( = 4) harbored Chikungunya virus (ChikV, Togaviridae), marking its first detection in these hosts. ( = 17) presented Coronaviridae and Orthoherpesviridae, while ( = 1) also carried Coronaviridae. A broad virome diversity, including Togaviridae and Adenoviridae members, was identified in ( = 43), with significant relevance to human health. Additional species, such as ( = 1), ( = 1), ( = 1), ( = 1), ( = 1), ( = 1), ( = 1), and ( = 1), contributed to insights into viral reservoirs. These results underscore the importance of virome studies in regions harboring high biodiversity, emphasizing genomic surveillance as a vital tool for monitoring zoonotic viruses and safeguarding global health.
在“同一个健康”框架内，监测野生动物病毒组对于识别动物源性病原体威胁至关重要。本研究采用宏基因组测序分析了巴西南部88只代表13个物种（猫科动物、野生啮齿动物、有袋动物和非人灵长类动物）的个体的直肠和口腔拭子。（=15只个体）和（=4只个体）携带基孔肯雅病毒（ChikV，披膜病毒科），这是该病毒在这些宿主中的首次检测。（=17只个体）呈现出冠状病毒科和正疱疹病毒科，而（=1只个体）也携带冠状病毒科。在（=43只个体）中鉴定出了广泛的病毒组多样性，包括披膜病毒科和腺病毒科成员，这与人类健康具有显著相关性。其他物种，如（=1只个体）、（=1只个体）、（=1只个体）、（=1只个体）、（=1只个体）、（=1只个体）、（=1只个体）和（=1只个体），有助于深入了解病毒宿主。这些结果强调了在生物多样性高的地区进行病毒组研究的重要性，强调基因组监测是监测动物源性病原体和保障全球健康的重要工具。
