巴西亚马逊地区蝙蝠体内病毒的鉴定：一项描述性宏基因组分析

Identification of Viruses in Bats from the Brazilian Amazon: A Descriptive Metagenomic Analysis.

作者信息

Pinheiro Lucas Rafael Santana, Rodrigues Érika Dayane Leal, Paiva Francisco Amilton Dos Santos, Cruz Ana Cecília Ribeiro, Medeiros Daniele Barbosa de Almeida, Casseb Alexandre do Rosário, Silva Sandro Patroca da, Casseb Livia Medeiros Neves

机构信息

Graduate Program in Virology, Hemorrhagic Fevers and Arbovirology Section (SAARB), Evandro Chagas Institute (IEC), BR-316 Highway, km 7, Levilândia, Ananindeua 67030-000, PA, Brazil.

Hemorrhagic Fevers and Arbovirology Section (SAARB), Evandro Chagas Institute (IEC), BR-316 Highway, km 7, Levilândia, Ananindeua 67030-000, PA, Brazil.

出版信息

Microorganisms. 2024 Mar 16;12(3):593. doi: 10.3390/microorganisms12030593.

DOI:10.3390/microorganisms12030593

PMID:38543644

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10974934/

Abstract

Bats are widely distributed in Brazil, including the Amazon region, and their association with viral pathogens is well-known. This work aimed to evaluate the metavirome in samples of sp. bats captured in the Brazilian Amazon from 2019 to 2021. Lung samples from 58 bats were divided into 13 pools for RNA isolation and sequencing followed by bioinformatic analysis. The family showed the highest abundance of viral reads. Although no complete genome could be recovered, the and families showed the formation of contigs with satisfactory identity and size characteristics for further analysis. One contig of the family was characterized as belonging to the genus , being grouped most closely phylogenetically to . The contig related to the family was identified within the genus, with 94%, 91%, and 42% amino acid identity with 2, , and , respectively. The presence of viruses in bats needs constant updating since the study was able to identify viral sequences related to families or genera still poorly described in the literature in association with bats.

摘要

蝙蝠广泛分布于巴西，包括亚马逊地区，并且它们与病毒病原体的关联是众所周知的。这项工作旨在评估2019年至2021年在巴西亚马逊地区捕获的种蝙蝠样本中的宏病毒组。来自58只蝙蝠的肺样本被分成13个池用于RNA分离和测序，随后进行生物信息学分析。科显示出病毒读数的丰度最高。尽管无法获得完整的基因组，但科和科显示出形成了具有令人满意的同一性和大小特征的重叠群，以便进行进一步分析。科的一个重叠群被鉴定为属于属，在系统发育上与关系最为密切。与科相关的重叠群在属内被鉴定出来，与 2、和的氨基酸同一性分别为94%、91%和42%。由于该研究能够鉴定出与文献中仍描述较少的与蝙蝠相关的科或属相关的病毒序列，因此蝙蝠中病毒的存在情况需要不断更新。