Bulatov Yerbol, Kurmasheva Alina, Amanova Zhanat, Abitaev Ruslan, Sametova Zhanna, Kyrgyzbayeva Asselya, Kondybaeva Zhanat, Turyskeldi Sholpan, Ussembay Abdurakhman, Toktyrova Dariya, Mazbayeva Dana, Shayakhmetov Yeraly, Kerimbayev Aslan, Khussainov Damir, Wentao Ma, Rsaliyev Aralbek, Abduraimov Yergali
Research Institute for Biological Safety Problems, National Holding "QazBioPharm", Guardeyskiy 080409, Kazakhstan.
Department of Veterinary and Zooengineering, Kazakh National Agrarian Research University, Almaty 050010, Kazakhstan.
Vaccines (Basel). 2025 Apr 15;13(4):408. doi: 10.3390/vaccines13040408.
: This article presents a study evaluating the antibody levels against infectious bovine rhinotracheitis (IBR) and bovine viral diarrhea (BVD) in Kazakh Whiteheaded calves born to dams immunized with an experimental inactivated combined vaccine against these infections. The vaccine formulation includes the strains "R-93" (IBR) and "Oregon CV" (BVD), which are preserved in the microorganism collection of the Research Institute for Biological Safety Problems. : To assess the immune response in newborn calves, blood serum samples were collected before the first intake of colostrum, followed by weekly sampling for 28 weeks post-birth. The antibody response was determined using a virus neutralization assay on MDBK cell cultures and lamb testicle cell cultures. : The results demonstrated that the protective antibody level against the IBR virus (≥2 log) persisted for up to 25 weeks, while the protective level against the BVD virus (≥3 log) remained for 23 weeks. Based on these findings, the vaccine was deemed safe, as it did not induce abortions or clinical manifestations of the diseases. The overall duration of the colostral immunity in calves against the IBR and BVD viruses reached 23 weeks. : Therefore, it is recommended that Kazakh Whiteheaded calves be vaccinated with the associated inactivated vaccine against infectious bovine rhinotracheitis and bovine viral diarrhea no earlier than 23 weeks of age.
本文介绍了一项研究，该研究评估了用针对传染性牛鼻气管炎（IBR）和牛病毒性腹泻（BVD）的实验性灭活联合疫苗免疫的母畜所生哈萨克白头犊牛体内针对这些感染的抗体水平。疫苗配方包括保存在生物安全问题研究所微生物保藏中心的“R - 93”株（IBR）和“俄勒冈CV”株（BVD）。
为了评估新生犊牛的免疫反应，在首次摄入初乳前采集血清样本，随后在出生后28周内每周采样一次。使用在MDBK细胞培养物和羔羊睾丸细胞培养物上进行的病毒中和试验来确定抗体反应。
结果表明，针对IBR病毒的保护性抗体水平（≥2 log）持续长达25周，而针对BVD病毒的保护性水平（≥3 log）持续23周。基于这些发现，该疫苗被认为是安全的，因为它不会引发流产或疾病的临床表现。犊牛针对IBR和BVD病毒的初乳免疫总持续时间达到23周。
因此，建议哈萨克白头犊牛在不早于23周龄时接种针对传染性牛鼻气管炎和牛病毒性腹泻的相关灭活疫苗。
