Butrón-Téllez Girón C, De Alba-Montero I, Hernández-Arteaga L O, Garrocho-Rangel A, Ruiz F
Faculty of Stomatology, Autonomous University of San Luis Potosí, Av. Dr. Manuel Nava No. 2, Zona Universitaria, C.P. 78290, San Luis Potosí, SLP, México.
Faculty of Sciences, Autonomous University of San Luis Potosí, Av. Parque Chapultepec 1570, Priv. del Pedregal, C.P. 78295, San Luis Potosí, SLP, México.
Eur Arch Paediatr Dent. 2025 May 7. doi: 10.1007/s40368-025-01048-z.
The aim of the present in vitro study was to synthesize and characterize green silver nanoparticles using Larrea tridentata leaf extract as a reducing and stabilizing agent and combining the nanoparticles with a commercial fluoride varnish (Fluor Protector) to evaluate the antibacterial activity in the production of acid and the adhesion of Streptococcus mutans and Lactobacillus acidophilus.
Silver nanoparticles were synthesized by green synthesis using Larrea tridentata leaf extract as a reducing and stabilizing agent and characterized by UV-Vis spectroscopy, dynamic light scattering (DLS), and transmission electron microscopy (TEM). Minimum inhibitory and bactericidal concentrations were determined using microdiluted plates. Antibacterial activity compared using disc diffusion. Adhesion and acidogenicity tested on deciduous teeth, divided into experimental (fluoride varnish with silver nanoparticles) and control (fluoride varnish) groups. Microorganism adhesion quantified on enamel, pH readings after 24-h incubation. Analyzed using ANOVA, Tukey's test, and Mann-Whitney U.
Fluoride varnish with silver nanoparticles showed better antibacterial activity than nanoparticles and varnish alone. Fluoride varnish with silver nanoparticles was more effective compared to fluoride varnish in inhibiting the adhesion and pH decreases of Streptococcus mutans and Lactobacillus acidophilus on the sound enamel, with statistically significant differences (p < 0.001).
Fluoride varnish with silver nanoparticles has a good antibacterial effect for inhibiting the adhesion and production of acid being is important in the prevention and the initial stages of dental caries.
本体外研究的目的是使用三齿拉瑞阿树叶提取物作为还原剂和稳定剂合成绿色银纳米颗粒，并将这些纳米颗粒与市售氟化物清漆（氟保护漆）结合，以评估其对产酸过程中的抗菌活性以及变形链球菌和嗜酸乳杆菌的黏附情况。
以三齿拉瑞阿树叶提取物作为还原剂和稳定剂，通过绿色合成法合成银纳米颗粒，并采用紫外-可见光谱、动态光散射（DLS）和透射电子显微镜（TEM）对其进行表征。使用微量稀释板测定最低抑菌浓度和杀菌浓度。采用纸片扩散法比较抗菌活性。在乳牙上测试黏附性和产酸性，乳牙分为实验组（含银纳米颗粒的氟化物清漆）和对照组（氟化物清漆）。对牙釉质上的微生物黏附进行定量分析，孵育24小时后读取pH值。采用方差分析、Tukey检验和Mann-Whitney U检验进行分析。
含银纳米颗粒的氟化物清漆比单独的纳米颗粒和清漆表现出更好的抗菌活性。与氟化物清漆相比，含银纳米颗粒的氟化物清漆在抑制变形链球菌和嗜酸乳杆菌在健康牙釉质上的黏附以及pH值下降方面更有效，差异具有统计学意义（p < 0.001）。
含银纳米颗粒的氟化物清漆在抑制黏附和产酸方面具有良好的抗菌效果，这在龋齿的预防和初始阶段很重要。
