Hashemikamangar Sedighe Sadat, Khadivi Moghadam Mohammadreza, Vahedi Mahtab, Rohaninasab Marzieh, Chiniforush Nasim
Department of Restorative Dentistry School of Dentistry Tehran University of Medical Sciences, Tehran, Iran.
Department of Surgical Sciences and Integrated Diagnostics University of Genoa, Viale Benedetto XV, Genoa, Italy.
Int J Dent. 2024 Aug 30;2024:6624453. doi: 10.1155/2024/6624453. eCollection 2024.
The aim of this study was to assess the color changes in artificially induced incipient caries after photodynamic therapy (PDT) using different concentrations of methylene blue and toluidine blue, along with irrigation using water and hypochlorite.
Forty-two sound human premolar teeth were used to create two artificial incipient carious lesions. One lesion was placed on the buccal surface and the other on the lingual surface. The color of these artificial incipient carious surfaces was determined using the CIE color system. The teeth were then randomly assigned to 12 groups ( = 7) based on the PDT method. These methods included methylene blue with concentrations of 50, 100, and 150 g/mL, followed by water irrigation, methylene blue with concentrations of 50, 100, and 150 g/mL, followed by hypochlorite solution irrigation, toluidine blue with concentrations of 50, 100, and 150 g/mL, followed by water irrigation, and toluidine blue with concentrations of 50, 100, and 150 g/mL, followed by hypochlorite solution irrigation. The teeth underwent a colorimetry procedure again, and the resulting color changes were calculated. A three-way ANOVA was performed to analyze the effects of laser wavelength, concentration of the light-absorbing material, and irrigation solution on .
The results showed that the color changes caused by toluidine blue photosensitizer at a concentration of 100 g/mL, with both water and hypochlorite irrigation, were not noticeable to the naked eye (water = 3.04, hypochlorite = 2.00). However, in the other study groups, the color changes were perceptible ( > 3.3). There were no significant differences in between different concentrations of methylene blue and toluidine blue when using either water or hypochlorite irrigation ( > 0.05). A significant difference was observed between methylene blue and toluidine blue at a concentration of 100 g/mL with water irrigation (=0.006). Additionally, a significant difference was found between methylene blue and toluidine blue at a concentration of 100 g/mL with hypochlorite irrigation (=0.049). However, no significant differences were observed between methylene blue and toluidine blue at other concentrations with either water or hypochlorite irrigation ( > 0.05).
In conclusion, tooth color in teeth with incipient caries did not change significantly after PDT using toluidine blue (the photosensitizer) at a concentration of 100 g/mL with either water or 1% hypochlorite solution irrigation for 5 s.
本研究旨在评估使用不同浓度的亚甲蓝和甲苯胺蓝进行光动力疗法（PDT）后，人工诱导的早期龋损的颜色变化，同时评估用水和次氯酸盐冲洗的效果。
使用42颗健康人前磨牙制造两个人工早期龋损。一个龋损位于颊面，另一个位于舌面。使用CIE颜色系统确定这些人工早期龋损表面的颜色。然后根据PDT方法将牙齿随机分为12组（每组n = 7）。这些方法包括浓度为50、100和150μg/mL的亚甲蓝，随后用水冲洗；浓度为50、100和150μg/mL的亚甲蓝，随后用次氯酸盐溶液冲洗；浓度为50、100和150μg/mL的甲苯胺蓝，随后用水冲洗；浓度为50、100和150μg/mL的甲苯胺蓝，随后用次氯酸盐溶液冲洗。牙齿再次进行比色程序，并计算产生的颜色变化。进行三因素方差分析以分析激光波长、吸光材料浓度和冲洗液对颜色变化的影响。
结果表明，浓度为100μg/mL的甲苯胺蓝光敏剂在用水和次氯酸盐冲洗后引起的颜色变化肉眼难以察觉（水冲洗= 3.04，次氯酸盐冲洗= 2.00）。然而，在其他研究组中，颜色变化是可察觉的（ΔE> 3.3）。当使用水或次氯酸盐冲洗时，不同浓度的亚甲蓝和甲苯胺蓝之间的颜色变化差异无统计学意义（P> 0.05）。在用水冲洗时，浓度为100μg/mL的亚甲蓝和甲苯胺蓝之间观察到显著差异（P = 0.006）。此外，在使用次氯酸盐冲洗时，浓度为100μg/mL的亚甲蓝和甲苯胺蓝之间也发现了显著差异（P = 0.049）。然而，在其他浓度下，用水或次氯酸盐冲洗时，亚甲蓝和甲苯胺蓝之间未观察到显著差异（P> 0.05）。
总之，对于早期龋损牙齿，使用浓度为100μg/mL的甲苯胺蓝（光敏剂）进行光动力疗法，随后用水或1%次氯酸盐溶液冲洗5秒后，牙齿颜色没有显著变化。
