State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, No.1295 Dingxi Road, Shanghai 200050, People's Republic of China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, No. 19(A) Yuquan Road, Beijing 100049, People's Republic of China.
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, No.1295 Dingxi Road, Shanghai 200050, People's Republic of China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, No. 19(A) Yuquan Road, Beijing 100049, People's Republic of China.
Acta Biomater. 2019 Dec;100:270-279. doi: 10.1016/j.actbio.2019.10.012. Epub 2019 Oct 10.
Developing multifunctional wound dressing with desired mechanical strength is of great significance for the treatment of different types of skin wounds. Inspired by the close relationship between strength and hierarchical structure of nacre, hierarchical and porous graphene oxide-chitosan-calcium silicate (GO-CTS-CS) film biomaterials are fabricated by a combination of vacuum filtration-assisted assembly and freeze-drying methods. The bioinspired hierarchical materials emulate an orderly porous lamellar micron-scale structure and the "brick-and-mortar"-layered nanostructure. The hierarchical microstructure endows the GO-CTS-CS biomaterials with good tensile strength, compatible breathability, and water absorption. Furthermore, the hierarchical GO-CTS-CS biomaterials exhibit ideal photothermal performance, leading to significant photothermal antibacterial and antitumor efficacy. Further, the hierarchical GO-CTS-CS biomaterials show stimulatory effect on in vivo chronic wound healing. Therefore, such a high performance and multifunctional biomaterial is believed to offer a promising alternative to traditional wound dressing in future. STATEMENT OF SIGNIFICANCE: Although it is an effective strategy to prepare high-performance materials by mimicking the hierarchical microstructure of nacre, the preparation of nacre-inspired materials in tissue engineering fields still needs to be investigated. In this work, we prepared a nacre-inspired multifunctional graphene oxide-chitosan-calcium silicate (GO-CTS-CS) biomaterial with a hierarchical microstructure. The hierarchical microstructure endows the biomaterials with desired properties of strength, breathability, and water absorption. Further, the hierarchical GO-CTS-CS biomaterial showed good photothermal antibacterial/antitumor and wound healing effects. This work may provide an approach to combine the preparation of multifunctional biomaterials with bioinspired engineering by constructing a hierarchical microstructure, indicating that the assembling hierarchical microstructure in biomaterials is of great importance for tissue engineering and regenerative medicine.
具有理想机械强度的多功能伤口敷料的开发对于治疗不同类型的皮肤伤口具有重要意义。受珍珠层强度与分级结构之间密切关系的启发，通过真空过滤辅助组装和冷冻干燥方法相结合，制备了分级多孔氧化石墨烯-壳聚糖-硅酸钙（GO-CTS-CS）膜生物材料。这种仿生分级材料模拟了有序多孔层状微米级结构和“砖-泥”层状纳米结构。分级微观结构使 GO-CTS-CS 生物材料具有良好的拉伸强度、透气性和吸水性。此外，分级 GO-CTS-CS 生物材料表现出理想的光热性能，导致显著的光热抗菌和抗肿瘤效果。此外，分级 GO-CTS-CS 生物材料对体内慢性伤口愈合具有刺激作用。因此，这种高性能多功能生物材料有望成为未来传统伤口敷料的一种有前途的替代品。
虽然通过模拟珍珠层的分级微观结构来制备高性能材料是一种有效的策略，但在组织工程领域中制备珍珠层启发材料仍需要进一步研究。在这项工作中，我们制备了一种具有分级微观结构的珍珠层启发多功能氧化石墨烯-壳聚糖-硅酸钙（GO-CTS-CS）生物材料。分级微观结构赋予了材料所需的强度、透气性和吸水性。此外，分级 GO-CTS-CS 生物材料表现出良好的光热抗菌/抗肿瘤和伤口愈合效果。这项工作可能为通过构建分级微观结构将多功能生物材料的制备与仿生工程相结合提供一种方法，表明在生物材料中组装分级微观结构对于组织工程和再生医学非常重要。
