Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuchang District, Wuhan 430072, Hubei Province, PR China.
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuchang District, Wuhan 430072, Hubei Province, PR China.
Int J Syst Evol Microbiol. 2023 Sep;73(9). doi: 10.1099/ijsem.0.006034.
A Gram-negative, yellow-pigmented, aerobic and rod-shaped bacterium, designated as strain BaP3, was isolated from the soil. Strain BaP3 grew at 16-37℃ (optimum, 30 °C) and pH 6.0-8.0 (optimum, pH 7.0). Additionally, strain BaP3 could tolerate NaCl concentrations in the range 0-6 % (optimum, 1%). Moreover, strain BaP3 was motile by flagella. The phylogenetic analysis of 16S rRNA sequences showed that strain BaP3 belonged to the genus , and the sequence was most closely related to CGMCC 1.3392 and DSM 15758, with 99.66 % sequence similarity. RY24 was the next closely related species, exhibiting 99.38 % 16S rRNA gene sequence similarity. The DNA-DNA hybridization and average nucleotide identity values between strain BaP3 and its closely related types were below 50 and 92 %, respectively. Both results were below the cut-off for species distinction. The genomic DNA G+C content of strain BaP3 was 65.30 mol%. The predominant quinone in strain BaP3 was identified as ubiquinone Q-9. The major cellular fatty acids were summed feature 8 (C 7 and/or C 6), summed feature 3 (C 7 and/or C 6) and C. These results indicated that strain BaP3 represents a novel species in the genus . The type strain is BaP3 (CCTCC AB 2022379=JCM 35914), for which the name sp. nov. is proposed.
一株革兰氏阴性、黄色、需氧、杆状细菌，被命名为 BaP3 菌株，从土壤中分离得到。BaP3 菌株在 16-37℃（最适温度 30℃）和 pH6.0-8.0（最适 pH7.0）条件下生长。此外，BaP3 菌株可以耐受 0-6%（最适 1%）的 NaCl 浓度。此外，BaP3 菌株通过鞭毛运动。16S rRNA 序列的系统发育分析表明，BaP3 菌株属于 属，与 CGMCC 1.3392 和 DSM 15758 的序列最密切相关，相似度为 99.66%。RY24 是下一个密切相关的物种，16S rRNA 基因序列相似度为 99.38%。BaP3 菌株与其密切相关类型之间的 DNA-DNA 杂交和平均核苷酸同一性值分别低于 50%和 92%，均低于物种区分的界限。菌株 BaP3 的基因组 DNA G+C 含量为 65.30mol%。BaP3 菌株中主要的醌类为泛醌 Q-9。主要的细胞脂肪酸为总和特征 8（C7 和/或 C6）、总和特征 3（C7 和/或 C6）和 C 。这些结果表明，BaP3 菌株代表 属中的一个新种。模式菌株为 BaP3（CCTCC AB 2022379=JCM 35914），提议将其命名为. sp. nov.。
