de Roode Lotte M, Sørensen Simon T, van der Stel Stefan D, de Boer Lisanne L, Wang Yineng, Lu Huihui, Andersson-Engels Stefan, Ruers Theo J M, Burke Ray
Netherlands Cancer Institute-Antoni van Leeuwenhoek, Amsterdam, The Netherlands.
Tyndall National Institute, University College Cork, IPIC, Biophotonics@Tyndall, Cork, Ireland.
J Biomed Opt. 2025 May;30(5):057001. doi: 10.1117/1.JBO.30.5.057001. Epub 2025 May 6.
We aim to validate the technologies essential for a handheld, optically guided biopsy device designed to enhance the diagnostic yield and accuracy of percutaneous liver biopsy procedures.
We aim to combine diffuse reflectance spectroscopy (DRS) and autofluorescence (AF) spectroscopy in a fiber-optic needle probe using mini spectrometers for the classification of tumor and healthy tissues.
A fiber-optic needle probe combining DRS and AF spectroscopy, incorporating mini spectrometers to facilitate future integration into a biopsy actuator, was designed and built. This custom probe was used to measure healthy liver tissues and colorectal metastases in excised liver segments. A linear discriminant analysis was applied to the DRS and AF data to distinguish tumors from healthy tissues.
The miniaturized combined DRS and AF spectroscopy system could accurately distinguish tumors from healthy liver with a sensitivity of 95% and a specificity of 96% from a sample size of patients and 52 measurements.
We demonstrate the feasibility of miniaturizing a combined DRS and AF spectroscopy system for the classification of tumor and healthy tissues. This validation supports the feasibility and further development of a handheld, optically guided biopsy device.
我们旨在验证一种手持式光学引导活检设备所必需的技术，该设备旨在提高经皮肝活检程序的诊断率和准确性。
我们旨在将漫反射光谱（DRS）和自发荧光（AF）光谱结合在一个光纤针式探头中，使用微型光谱仪对肿瘤组织和健康组织进行分类。
设计并制造了一种结合DRS和AF光谱的光纤针式探头，该探头集成了微型光谱仪，以便将来能够集成到活检驱动器中。这个定制探头用于测量切除肝段中的健康肝组织和结直肠转移瘤。对DRS和AF数据应用线性判别分析，以区分肿瘤组织和健康组织。
小型化的DRS和AF光谱组合系统能够准确区分肿瘤组织和健康肝组织，在对 名患者进行52次测量的样本量下，灵敏度为95%，特异性为96%。
我们证明了将DRS和AF光谱组合系统小型化用于肿瘤组织和健康组织分类的可行性。这一验证支持了手持式光学引导活检设备的可行性和进一步开发。
