Fakhim Harmed, Mohamadi Bahar, Gharibi Shima, Rahimmalek Medhi, Hosseini Rizi Mahnaz, Shelerangkon Mahsa, Nasri Elahe, Dorostkar Fariba, Szumny Antoni, Vaezi Afsane
Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Microbiology, Tehran North Branch, Islamic Azad University, Tehran, Iran.
Iran J Microbiol. 2025 Apr;17(2):342-347. doi: 10.18502/ijm.v17i2.18398.
The rapid emergence of resistant fungi is occurring worldwide, and this crisis has been attributed to the lack of new antifungal drug development. This issue emphasizes the need for innovation in finding novel antifungals. There is an increasing interest in using the natural products of plants with high biological activity as alternatives to synthetic drugs. This study aimed to evaluate the possible applicability of polyphenols as alternative antifungal drugs to treat resistant infections.
A panel of fluconazole-resistant (n=14) and fluconazole-susceptible (n=26) clinical isolates was obtained from the reference culture collection. The determination of the minimum inhibitory concentrations (MICs) of fluconazole, tannic acid, rosmarinic acid, gallic acid, chlorogenic acid, caffeic, ferulic, and p-coumaric was carried out following the Clinical and Laboratory Standards Institute (CLSI) guidelines.
The MIC values of 40 species isolates ranged from 0.25 to >64 μg/mL for polyphenolic compounds. The highest inhibitory effect against species was observed with tannic acid, followed by fluconazole. Non- groups were more sensitive to tannic acid compared to isolates. Significant differences were observed in the MICs of fluconazole and tannic acid against non- isolates.
The increasing antifungal resistance highlights the importance of evaluating new drugs that are more robust against resistance. This study suggests that tannic acid could be considered a novel antifungal agent for managing fungal infections, including multidrug-resistant non- infections.
耐药真菌在全球范围内迅速出现，这场危机归因于新型抗真菌药物研发的匮乏。这一问题凸显了寻找新型抗真菌药物时创新的必要性。人们越来越有兴趣将具有高生物活性的植物天然产物用作合成药物的替代品。本研究旨在评估多酚类作为替代抗真菌药物治疗耐药感染的可能适用性。
从参考培养物保藏中心获得一组氟康唑耐药（n = 14）和氟康唑敏感（n = 26）的临床分离株。按照临床和实验室标准协会（CLSI）指南测定氟康唑、单宁酸、迷迭香酸、没食子酸、绿原酸、咖啡酸、阿魏酸和对香豆酸的最低抑菌浓度（MIC）。
40种分离株对多酚类化合物的MIC值范围为0.25至>64μg/mL。单宁酸对分离株的抑制作用最强，其次是氟康唑。与分离株相比，非组对单宁酸更敏感。氟康唑和单宁酸对非分离株的MIC存在显著差异。
日益增加的抗真菌耐药性凸显了评估对耐药性更具抗性的新药的重要性。本研究表明，单宁酸可被视为一种新型抗真菌剂，用于治疗真菌感染，包括多重耐药非感染。
