Hosseini Masoumeh Sadat, Navidinia Masoumeh, Seyedjavadi Sima Sadat, Goudarzi Mehdi, Rasouli Helia, Mahdavian Amir Mohsen, Zamani Elina Rahimi
Department of Microbial Biotechnology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran.
Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2025 Apr;17(2):293-302. doi: 10.18502/ijm.v17i2.18395.
The azole antifungals are the most frequent class used to treat infections. It is essential to elucidate the potential of natural compounds as an alternative in eliminating Therefore, in the present study, the antagonistic effect of toxins on azole antifungal resistance in species was investigated.
In this study, 28 species with azole antifungal resistance were obtained from patients at Shohadaye Tajrish Hospital. The effect of toxins, such as phenazine, pyocyanin, pyoverdine, and fluorescein, was examined on species. The antifungal activity of these toxins against spp. was determined using methods such as minimal inhibitory concentration (MIC ), radial diffusion assay (RDA), and detection of reactive oxygen species (ROS).
The prevalence of strains in urinary catheters, surgical wounds, respiratory tracts, blood, and standard strains was 46.3%, 21.4%, 25%, 7.14%, and 3.57%, respectively. The MIC values were reported as 32 μg/ml for phenazine, and 128 μg/ml for pyoverdine, pyocyanin, and fluorescein. The results showed that phenazine exhibited higher inhibitory effects against isolated from clinical samples compared to the other toxins. After exposure to phenazines (20 μg/ml), 65-70% of yeast cells of spp. showed rhodamine 123 fluorescence, indicating high intracellular reactive oxygen species (ROS) production.
The antifungal effect of different toxins in spp. may be due to ROS-mediated apoptotic death. The results suggest that phenazine has high potential in controlling . This natural compounds are a potential alternative for eliminating this yeast.
唑类抗真菌药物是治疗感染最常用的一类药物。阐明天然化合物作为消除感染的替代物的潜力至关重要。因此，在本研究中，研究了毒素对念珠菌属物种唑类抗真菌耐药性的拮抗作用。
在本研究中，从塔吉里什烈士医院的患者中获得了28株对唑类抗真菌药物耐药的念珠菌属物种。检测了吩嗪、绿脓菌素、绿脓杆菌荧光素和荧光素等毒素对念珠菌属物种的作用。使用最小抑菌浓度（MIC）、径向扩散试验（RDA）和活性氧（ROS）检测等方法测定了这些毒素对念珠菌属物种的抗真菌活性。
导尿管、手术伤口、呼吸道、血液和标准菌株中念珠菌属菌株的患病率分别为46.3%、21.4%、25%、7.14%和3.57%。吩嗪的MIC值报告为32μg/ml，绿脓杆菌荧光素、绿脓菌素和荧光素的MIC值为128μg/ml。结果表明，与其他毒素相比，吩嗪对从临床样本中分离出的念珠菌属具有更高的抑制作用。暴露于吩嗪（20μg/ml）后，65-70%的念珠菌属酵母细胞显示罗丹明123荧光，表明细胞内活性氧（ROS）产生量高。
不同毒素对念珠菌属物种的抗真菌作用可能是由于ROS介导的凋亡死亡。结果表明，吩嗪在控制念珠菌属方面具有很高的潜力。这种天然化合物是消除这种酵母的潜在替代物。
