Institute of Natural Resources and Agrobiology of Seville, Spanish National Research Council (IRNAS-CSIC), 41012 Seville, Spain.
J Hazard Mater. 2024 Feb 15;464:132970. doi: 10.1016/j.jhazmat.2023.132970. Epub 2023 Nov 10.
Ibuprofen (IBP) is a widely used drug of environmental concern as emerging contaminant due to its low elimination rates by wastewater treatment plants (WWTPs), leading to the contamination of the environment, where IBP is introduced mainly from wastewater discharge and sewage sludge used as fertilizer. This study describes the application of a consortium from sewage sludge and acclimated with ibuprofen (consortium C7) to accelerate its biodegradation both in solution and sewage sludge. 500 mg L IBP was degraded in solution in 28 h, and 66% mineralized in 3 days. IBP adsorbed in sewage sludge (10 mg kg) was removed after bioaugmentation with C7 up to 90% in 16 days, with a 5-fold increase in degradation rate. This is the first time that bioaugmentation with bacterial consortia or isolated bacterial strains have been used for IBP degradation in sewage sludge. The bacterial community of consortium C7 was significantly enriched in Sphingomonas wittichii, Bordetella petrii, Pseudomonas stutzeri and Bosea genosp. after IBP degradation, with a special increase in abundance of S. wittichii, probably the main potential bacterial specie responsible for IBP mineralization. Thirteen bacterial strains were isolated from C7 consortium. All of them degraded IBP in presence of glucose, especially Labrys neptuniae. Eight of these bacterial strains (B. tritici, L. neptuniae, S. zoogloeoides, B. petrii, A. denitrificans, S. acidaminiphila, P. nitroreducens, C. flaccumfaciens) had not been previously described as IBP-degraders. The bacterial community that makes up the indigenous consortium C7 appears to have a highly efficient biotic degradation potential to facilitate bioremediation of ibuprofen in contaminated effluents as well as in sewage sludge generated in WWTPs.
布洛芬（IBP）是一种广泛使用的药物，由于其在废水处理厂（WWTP）中的低去除率，作为新兴污染物引起了关注，导致环境受到污染，IBP 主要通过废水排放和用作肥料的污水污泥进入环境。本研究描述了一种由污水污泥组成的联合体（C7 联合体）的应用，该联合体经过布洛芬驯化，可加速其在溶液和污水污泥中的生物降解。500mg/L 的 IBP 在溶液中 28 小时内降解，3 天内 66%矿化。生物强化后，C7 可去除吸附在污水污泥（10mg/kg）中的 IBP，16 天内去除率达 90%，降解速率提高了 5 倍。这是首次使用细菌联合体或分离的细菌菌株在污水污泥中进行 IBP 降解的生物强化。在 IBP 降解后，C7 联合体的细菌群落中 Sphingomonas wittichii、Bordetella petrii、Pseudomonas stutzeri 和 Bosea genosp. 的丰度显著增加，特别是 S. wittichii 的丰度增加特别明显，可能是负责 IBP 矿化的主要潜在细菌。从 C7 联合体中分离出 13 株细菌。所有这些细菌在葡萄糖存在的情况下都能降解 IBP，特别是 Labrys neptuniae。其中 8 株细菌（B. tritici、L. neptuniae、S. zoogloeoides、B. petrii、A. denitrificans、S. acidaminiphila、P. nitroreducens、C. flaccumfaciens）以前没有被描述为 IBP 降解菌。构成土著 C7 联合体的细菌群落似乎具有高效的生物降解潜力，可促进受污染废水中布洛芬以及 WWTP 产生的污水污泥的生物修复。
