Irukuvarjula Vishalakshi, Fouladgar Faye, Powell Robert, Carney Emily, Habibi Neda
Department of Biomedical Engineering, University of North Texas, Denton, TX, United States.
OpenNano. 2025 Jan;21. doi: 10.1016/j.onano.2024.100223. Epub 2024 Dec 4.
There is a pressing need for new cell-laden, printable bioinks to mimic stiffer tissues such as cartilage, fibrotic tissue and bone. PEGDA monomers are bioinks that crosslink with light to form a viscoelastic solid, however, they lack cell adhesion properties. Here, we utilized a hybrid bioink by combining self-assembled peptide nanofibers with PEGDA for 3D printing lumens. Adult human dermal fibroblast (aHDF) cells were first seeded in peptide-laden in 2D and 3D layers and cell behavior were studied. The cell's morphology remained spheres when they were infused in the 3D hydrogel and highly aligned with 2D overlay hydrogels. HDF cells did not adhere to unmodified PEGDA lumens, however, they successfully attached and proliferated on PEGDA/peptide lumens. Moreover, HDF cells seeded on the hybrid PEGDA/peptide lumens displayed a distinct spread F-actin morphology. The results showcase the potential of peptide hydrogels in facilitating interaction of anchorage dependent cells with PEGDA structures.
迫切需要新型的载细胞、可打印生物墨水来模拟诸如软骨、纤维化组织和骨骼等更坚硬的组织。聚乙二醇二丙烯酸酯（PEGDA）单体是通过光交联形成粘弹性固体的生物墨水，然而，它们缺乏细胞粘附特性。在此，我们通过将自组装肽纳米纤维与PEGDA相结合来制备用于3D打印管腔的混合生物墨水。首先将成人人类真皮成纤维细胞（aHDF）接种在载有肽的二维和三维层中，并研究细胞行为。当细胞注入三维水凝胶中时，其形态保持球形，而在二维覆盖水凝胶中高度排列。HDF细胞不粘附于未修饰的PEGDA管腔，然而，它们成功地附着并在PEGDA/肽管腔上增殖。此外，接种在混合PEGDA/肽管腔上的HDF细胞呈现出明显的铺展F-肌动蛋白形态。结果展示了肽水凝胶在促进锚定依赖性细胞与PEGDA结构相互作用方面的潜力。
