Sultana Robina, Wang Xiukang, Azeem Muhammad, Hussain Tabassum, Mahmood Athar, Fiaz Sajid, Qasim Muhammad
Biosaline Research Laboratories, Department of Botany, University of Karachi, Karachi, Pakistan.
College of Life Sciences, Yan'an University, Yan'an, China.
Front Plant Sci. 2022 Apr 6;13:799404. doi: 10.3389/fpls.2022.799404. eCollection 2022.
Secondary metabolites, such as phenolic compounds, play an important role in alleviating salinity-induced negative effects in plants. The present study focused on seed priming and foliar application of a potent phenolic compound, coumarin, to induce salinity tolerance in var. SS-77. Based on pilot experiment, 100 mg L concentration of coumarin was applied to mitigate the negative effects of salinity on Sorghum, grown at 0, 100, and 200 mM NaCl under netted greenhouse conditions. Coumarin was applied to each salinity treatment in four different ways (i) non-primed control (NP), (ii) seed priming (COP), (iii) foliar application (COF), and (iv) a combination of seed priming and foliar application (COPF). Salinity stress significantly reduced the plant growth, biochemical attributes, and photosynthetic efficiency of Sorghum, whereas coumarin treatments (COP, COF, and COPF) showed a significant increase (P< 0.01) in above-mentioned parameters at all salinities. Among all, the combined treatment (COPF) showed maximum increase in growth, biochemicals, photosynthetic pigments, antioxidant enzymes, and photosynthetic efficiency parameters. Therefore, it is suggested that a combination of seed priming and foliar spray of 10 mg L coumarin is more suitable than their individual applications. It is an environment friendly and economically feasible approach that will be used to improve salinity tolerance of Sorghum and helpful to get considerable biomass from saline degraded lands to fulfill food, fodder, and energy demands of the ever-growing population.
次生代谢产物，如酚类化合物，在减轻盐分对植物造成的负面影响方面发挥着重要作用。本研究聚焦于用一种强效酚类化合物香豆素进行种子引发和叶面喷施，以诱导SS - 77品种高粱的耐盐性。基于预试验，在网状温室条件下，采用100 mg/L浓度的香豆素减轻盐分对生长在0、100和200 mM NaCl环境下高粱的负面影响。香豆素以四种不同方式应用于每种盐分处理：(i) 未引发对照 (NP)，(ii) 种子引发 (COP)，(iii) 叶面喷施 (COF)，以及 (iv) 种子引发和叶面喷施相结合 (COPF)。盐分胁迫显著降低了高粱的植株生长、生化特性和光合效率，而香豆素处理（COP、COF和COPF）在所有盐度下均使上述参数显著增加（P < 0.01）。其中，联合处理（COPF）在生长、生化物质、光合色素、抗氧化酶和光合效率参数方面增加最多。因此，建议10 mg/L香豆素的种子引发和叶面喷施相结合比单独应用更合适。这是一种环境友好且经济可行的方法，将用于提高高粱的耐盐性，并有助于从盐碱退化土地获取可观的生物量，以满足不断增长的人口对食物、饲料和能源的需求。
