Maranesi Alessia, Mohammadi Sajad, Castañon Ismael, Gama-Franceschi Felipe, Falciani Chiara, Pini Alessandro, Mezzanotte Laura, Unger Wendy, Ferrari Aldo
Department of Material Science and Engineering, Universitat Politècnica de Catalunya, Barcelona, 08019, Spain.
Hylomorph AG, Technopark, Zurich, 8005, Switzerland.
Sci Rep. 2025 May 10;15(1):16353. doi: 10.1038/s41598-025-99808-w.
The physical and chemical properties of implanted materials play a key role in their interaction with synthetic peptides that exert antimicrobial activity. In this study, we explored the diffusion properties and efficacy of the SET-M33 antimicrobial peptide in combination with artificial substrates, comprising cardiac implantable electronic devices (CIEDs) or porous protective envelopes. We found that porous materials, such as biosynthesized cellulose, polymeric meshes, and electrospun membranes, were conducive to SET-M33 diffusion. The diffusion dynamics was controlled by the intrinsic fibrous architecture of the materials. Biosynthesized cellulose supported the peptide's antimicrobial activity against E. coli and S. aureus. The efficacy of SET-M33 was instead reduced when combined with the other tested porous membranes and non-porous CIED interfaces, such as titanium and silicone. On the other hand, the low porosity of biosynthesized cellulose membranes, while effective in retaining the drug, diminished diffusion and thus peptide availability. In light of these findings, the implications for the use of antimicrobial peptides in the prevention of CIED surgical pocket infections are discussed.
植入材料的物理和化学性质在其与具有抗菌活性的合成肽的相互作用中起着关键作用。在本研究中，我们探讨了SET-M33抗菌肽与包括心脏植入式电子设备（CIED）或多孔保护包膜在内的人工基质结合时的扩散特性和功效。我们发现，诸如生物合成纤维素、聚合物网和电纺膜等多孔材料有利于SET-M33的扩散。扩散动力学受材料固有的纤维结构控制。生物合成纤维素支持该肽对大肠杆菌和金黄色葡萄球菌的抗菌活性。相反，当SET-M33与其他测试的多孔膜和无孔CIED界面（如钛和硅酮）结合时，其功效会降低。另一方面，生物合成纤维素膜的低孔隙率虽然能有效保留药物，但会减少扩散，从而降低肽的可用性。鉴于这些发现，我们讨论了抗菌肽在预防CIED手术囊袋感染中的应用意义。
