Iyer Divya, Laws Eric, LaJeunesse Dennis
Department of Nanoscience, Joint School of Nanoscience and Nanoengineering, University of North Carolina Greensboro, 2907 East Lee Street, Greensboro, North Carolina 27455, United States.
ACS Omega. 2023 Oct 9;8(50):47520-47529. doi: 10.1021/acsomega.3c04747. eCollection 2023 Dec 19.
Biofilm formation is a multistep process that requires initial contact between a bacterial cell and a surface substrate. Recent work has shown that nanoscale topologies impact bacterial cell viability; however, less is understood about how nanoscale surface properties impact other aspects of bacterial behavior. In this study, we examine the adhesive, viability, morphology, and colonization behavior of the bacterium on 21 plasma-etched polymeric surfaces. Although we predicted that specific nanoscale surface structures of the surface would control specific aspects of bacterial behavior, we observed no correlation between any bacterial response or surface structures/properties. Instead, it appears that the surface composition of the polymer plays the most significant role in controlling and determining a bacterial response to a substrate, although changes to a polymeric surface via plasma etching alter initial bacteria colonization and morphology.
生物膜形成是一个多步骤过程，需要细菌细胞与表面基质之间进行初始接触。最近的研究表明，纳米级拓扑结构会影响细菌细胞的活力；然而，对于纳米级表面特性如何影响细菌行为的其他方面，人们了解较少。在本研究中，我们研究了细菌在21种等离子体蚀刻聚合物表面上的粘附、活力、形态和定殖行为。尽管我们预测表面特定的纳米级表面结构会控制细菌行为的特定方面，但我们未观察到任何细菌反应与表面结构/特性之间存在相关性。相反，似乎聚合物的表面组成在控制和确定细菌对基质的反应中起最重要作用，尽管通过等离子体蚀刻对聚合物表面进行的改变会改变初始细菌定殖和形态。
