Taponaĭnen N Ia, Gotlib V Ia
Radiobiologiia. 1985 Jul-Aug;25(4):515-7.
In experiments on asynchronous population of HeLa S3 cells a study was made of the possibility of assessing DNA lesions which remained unrepaired for a long period of time following gamma-irradiation: in generation "O" directly affected by radiation and in generation "I" following the irradiated one. The presence of DNA damages was estimated by the reduction in survival of exposed cells incubated with inhibitors of repair and replicative syntheses of DNA, namely, with arabinoside cytosine and hydroxyurea. A considerable enhancement of the radiation effect was noted with the inhibitors added 0-6 h after irradiation (generation "O"), and a marked increase in the cell death was registered with the preparations injected 24-30 h after exposure (generation "I"). It is assumed that minor residual lesions persist in the generation of cells, following the one directly affected by gamma-radiation, which have completed the first postirradiation mitosis.
在对HeLa S3细胞异步群体进行的实验中，研究了评估γ射线照射后长时间未修复的DNA损伤的可能性：在受辐射直接影响的“O”代以及受辐射后的“I”代中进行评估。通过用DNA修复和复制合成抑制剂（即阿糖胞苷和羟基脲）孵育的受照射细胞存活率的降低来估计DNA损伤的存在。在照射后0 - 6小时添加抑制剂（“O”代）时，观察到辐射效应显著增强，而在照射后24 - 30小时注射制剂时（“I”代），细胞死亡明显增加。据推测，在受γ射线直接影响的细胞代之后，完成首次照射后有丝分裂的细胞代中存在轻微的残留损伤。
