Iswara Raja Afw, Hestiantoro Andon, Budiningsih Yuli, Werdhani Retno A, Birowo Ponco, Wuyung Puspita E, Fadilah Fadilah, Afandi Dedi
Doctoral Program in Medical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Department of Forensic and Medicolegal, Faculty of Medicine, Universitas Halu Oleo, Kendari, Indonesia.
Narra J. 2025 Apr;5(1):e1256. doi: 10.52225/narra.v5i1.1256. Epub 2025 Feb 5.
Sexual violence, including sexual intercourse, can occur in women experiencing vaginal discharge, particularly in cases of vaginal candidiasis. In candidiasis, the vaginal microbiome undergoes changes that could serve as a diagnostic indicator or as evidence of sexual activity. The aim of this study was to assess the effects of semen insemination on the vaginal microbiome profile of candidiasis rats and to determine its forensic investigations in cases of sexual violence. An experimental study was carried out using Wistar strain rats consisting of four male rats (for spermatozoa donors) and twenty-four female rats. The female rats were divided into four groups: normal condition (control), normal condition post-semen insemination, candidiasis rats, and candidiasis rat post-semen insemination. Vaginal microbiome profiles were examined for each group, using alpha diversity (Chao 1, Shannon, Simpson, and Faith PD indices) and beta diversity (Bray Curtis, Jaccard, Unweighted Unifrac and Weighted Unifrac indices). Data were analyzed using the Kruskal-Wallis test for alpha diversity and the PERMANOVA test for beta diversity. There is no significant difference in alpha and beta diversity of the vaginal microbiome between groups of rats in normal conditions and those with candidiasis, both pre- and post-semen insemination (>0.05). In candidiasis rats, the microbiome predominantly consisted of the Eukaryota kingdom, particularly . However, the study highlights that the presence of , and Archaea in the vaginal microbiome post-semen insemination potentially serves as an indicator of sexual intercourse, which may provide evidence of sexual violence in forensic medicine.
性暴力，包括性交，可能发生在有阴道分泌物的女性身上，尤其是在阴道念珠菌病的情况下。在念珠菌病中，阴道微生物群会发生变化，这些变化可作为诊断指标或性活动的证据。本研究的目的是评估精液授精对念珠菌病大鼠阴道微生物群谱的影响，并确定其在性暴力案件中的法医调查作用。使用Wistar品系大鼠进行了一项实验研究，包括四只雄性大鼠（作为精子供体）和二十四只雌性大鼠。雌性大鼠分为四组：正常状态（对照组）、精液授精后的正常状态、念珠菌病大鼠以及精液授精后的念珠菌病大鼠。使用α多样性（Chao 1、香农、辛普森和费思PD指数）和β多样性（布雷-柯蒂斯、雅卡尔、非加权 UniFrac 和加权 UniFrac 指数）对每组的阴道微生物群谱进行检查。使用Kruskal-Wallis检验分析α多样性数据，使用PERMANOVA检验分析β多样性数据。在正常状态的大鼠组和念珠菌病大鼠组中，无论精液授精前后，阴道微生物群的α和β多样性均无显著差异（>0.05）。在念珠菌病大鼠中，微生物群主要由真核生物界组成，尤其是 。然而，该研究强调，精液授精后阴道微生物群中 、古菌的存在可能作为性交的指标，这可能为法医学中的性暴力提供证据。
