Chen Libin, Li Haiwei, Liu Jing, Wang Yunzhong, Zhang Shengmin
Liaoning Cancer Hospital & Institute, Cancer Hospital of Dalian University of Technology, Cancer Hospital of China Medical University, Shenyang 110042, China.
Department of Ultrasound Medicine, The First Affiliated Hospital of Ningbo University, Ningbo 315010, China.
Cyborg Bionic Syst. 2025 May 9;6:0247. doi: 10.34133/cbsystems.0247. eCollection 2025.
Sono-immunotherapy is expected to effectively enhance treatment efficacy and reduce mortality in patients with pancreatic cancer. Hence, efficient applicable sono-immunotherapy systems are urgently needed for the treatment of this condition. In this study, hollow mesoporous carbon (HMC) nanoparticles were prepared using the sacrificial template method. These nanoparticles had a porphyrin-like structure and could generate singlet oxygen more efficiently than commercial TiO. Cellular assays showed that HMC killed tumor cells in the presence of ultrasonication, primarily by inducing apoptosis. HMC could also accelerate the release of immune factors by tumor cells, thereby activating dendritic cells and enhancing the efficacy of immunotherapy. Experiments in tumor-bearing mice and in situ pancreatic cancer tests showed that HMC, in combination with the small-molecule inhibitors of programmed cell death ligand 1, could reduce tumor growth via the generation of reactive oxygen species following ultrasonication. HMC could enhance the efficacy of immunotherapy by disrupting the immunosuppressive tumor microenvironment and promoting the accumulation of immune cells. Accordingly, in vivo sono-immunotherapy was achieved, and the growth of transplanted tumors and in situ tumors could be reduced. In conclusion, this study proposes a novel method for the preparation of HMC nanoparticles and demonstrates their potential in tumor treatment. Additionally, owing to their unique structure, these HMC nanoparticles could be used for different combination therapies tailored based on specific clinical requirements.
声动力免疫疗法有望有效提高胰腺癌患者的治疗效果并降低死亡率。因此，迫切需要高效适用的声动力免疫治疗系统来治疗这种疾病。在本研究中，采用牺牲模板法制备了中空介孔碳（HMC）纳米颗粒。这些纳米颗粒具有类卟啉结构，比市售的TiO更有效地产生单线态氧。细胞实验表明，HMC在超声作用下杀死肿瘤细胞，主要是通过诱导细胞凋亡。HMC还可以加速肿瘤细胞释放免疫因子，从而激活树突状细胞并增强免疫治疗效果。在荷瘤小鼠体内和原位胰腺癌实验表明，HMC与程序性细胞死亡配体1的小分子抑制剂联合使用，可以通过超声作用后产生活性氧来减少肿瘤生长。HMC可以通过破坏免疫抑制性肿瘤微环境和促进免疫细胞的积累来增强免疫治疗效果。因此，实现了体内声动力免疫治疗，并可减少移植瘤和原位瘤的生长。总之，本研究提出了一种制备HMC纳米颗粒的新方法，并证明了它们在肿瘤治疗中的潜力。此外，由于其独特的结构，这些HMC纳米颗粒可用于根据特定临床需求定制的不同联合治疗。
