Shanghai Tenth People's Hospital, School of Medicine, Tongji University Cancer Center, Tongji University, Shanghai, 200092, China.
School of Medical Technology, School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
J Nanobiotechnology. 2022 Dec 1;20(1):507. doi: 10.1186/s12951-022-01715-z.
Introducing oxygen-vacancy into the surface of the non-enzymatic sensor is supposed to be an effective way to improve inherently low catalytic activity and specificity of non-enzymatic sensors. In this work, CuO/C was synthesized at different temperatures using metal-organic frameworks as sacrificial templates to receive additional content of oxygen-vacancy. The product with the highest oxygen vacancy was found at 400 °C (named CuO/C-400 °C), which increased catalytically active sites and enhanced the charge-transfer efficiency. The sensing performance was afterward explored by amperometry under an optimal applied potential at 0.5 V (vs. SCE), presenting a broad detection range from 5.0 µM to 25.325 mM (R = 0.9998) with a sensitivity of 244.71 µA mM cm, and a detection limit of 1 µM. Furthermore, the reliability and selectivity of CuO/C-400 °C sensors were extensively explored in the presence of artificial serum/saliva samples with gradient glucose concentrations. The human blood samples were also detected with high recoveries compared with the clinical Hexokinase method. Hence, the prepared CuO/C-400 °C sensor with a broad detection range and high selectivity can be applied for the diabetes diagnosis ex vivo without further dilution for real-time analysis in practical applications.
在非酶传感器的表面引入氧空位被认为是提高非酶传感器固有低催化活性和特异性的有效方法。在这项工作中，使用金属有机骨架作为牺牲模板，在不同温度下合成了 CuO/C，以获得额外的氧空位含量。在 400°C（命名为 CuO/C-400°C）下得到的产物具有最高的氧空位，增加了催化活性位点并提高了电荷转移效率。随后在最佳施加电位 0.5 V（相对于 SCE）下通过电流法进行了传感性能研究，在 5.0 µM 至 25.325 mM（R=0.9998）的宽检测范围内呈现出 244.71 µA mM cm 的灵敏度和 1 µM 的检测限。此外，在含有梯度葡萄糖浓度的人工血清/唾液样本存在的情况下，广泛研究了 CuO/C-400°C 传感器的可靠性和选择性。还与临床己糖激酶法相比，对人血样进行了高回收率检测。因此，制备的 CuO/C-400°C 传感器具有宽检测范围和高选择性，可用于无需进一步稀释即可进行实时分析的实际应用中的体外糖尿病诊断。
