Ma Haiyan, Ren Zhitong, Luo Aihua, Fang Xiaoting, Liu Ruilin, Wu Chao, Shi Xinxin, Li Junji, Lv Heping, Sun Xiaohua, Zhang Kaiqin, Zheng Shunlin
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China; Crop Ecophysiology and Cultivation Key Laboratory of Sichuan Province, Chengdu 611130, China; Yibin Academy of Agricultural Sciences, Yibin 644699, China.
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China; Crop Ecophysiology and Cultivation Key Laboratory of Sichuan Province, Chengdu 611130, China.
Plant Commun. 2025 Jul 14;6(7):101372. doi: 10.1016/j.xplc.2025.101372. Epub 2025 May 12.
Continuous-cropping (CC) obstacles are among the primary factors limiting the development of global agriculture. Although most plants are negatively affected by barriers that develop with CC, they may also overcome such obstacles by altering the soil biological and chemical environment to favor plant growth. In this study, we investigated the mechanism by which plants recruit beneficial microorganisms using root exudates to alleviate obstacles in a 10-year CC potato system. On day 20 after potato emergence, soil microorganisms in the CC system promoted an increase in adventitious root (AR) numbers by increasing the indole-3-acetic acid (IAA) content of the rhizosphere soil. Analysis of rhizosphere bacterial communities using 16S rRNA sequencing revealed that CC alters community structure, increasing the abundance of Pantoea sp. MCC16. Irrigation with root exudates from CC potato significantly increased AR numbers and Pantoea sp. MCC16 abundance. Through untargeted metabolomic analysis, we identified nobiletin as key metabolite that promotes Pantoea sp. MCC16 colonization in the rhizosphere. Furthermore, application of either nobiletin or Pantoea sp. MCC16 significantly improved the yield of CC potatoes. These findings demonstrate that CC plants can actively secrete the unique metabolite nobiletin to recruit Pantoea sp. MCC16, a high IAA producer, to help plants recover functional traits and mitigate CC obstacles.
连作障碍是限制全球农业发展的主要因素之一。尽管大多数植物会受到连作产生的障碍的负面影响，但它们也可能通过改变土壤生物和化学环境以利于植物生长来克服这些障碍。在本研究中，我们调查了植物利用根系分泌物招募有益微生物以缓解10年连作马铃薯系统障碍的机制。马铃薯出苗后第20天，连作系统中的土壤微生物通过增加根际土壤中吲哚-3-乙酸（IAA）的含量，促进不定根数量增加。利用16S rRNA测序分析根际细菌群落表明，连作会改变群落结构，增加泛菌属MCC16的丰度。用连作马铃薯的根系分泌物灌溉显著增加了不定根数量和泛菌属MCC16的丰度。通过非靶向代谢组学分析，我们确定川陈皮素是促进泛菌属MCC16在根际定殖的关键代谢物。此外，施用川陈皮素或泛菌属MCC16均显著提高了连作马铃薯的产量。这些发现表明，连作植物可以主动分泌独特的代谢物川陈皮素，以招募高IAA生产者泛菌属MCC16，帮助植物恢复功能性状并减轻连作障碍。
