Flichman Diego M, Marquez Nelson, Pereson Matías Javier, Sánchez S Victor A, Gómez de la Fuente Andrea S, González Cecilia, Lema José Martín, Espíndola Sonia L, Carballo Graciela M, Martínez Alfredo P, Baré Patricia, Di Lello Federico A
Instituto de Investigaciones Biomédicas en Retrovirus y Síndrome de Inmunodeficiencia Adquirida (INBIRS), Universidad de Buenos Aires, Buenos Aires, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
J Med Virol. 2025 May;97(5):e70388. doi: 10.1002/jmv.70388.
The rise of reemerging pathogens such as DENV and CHIKV presents a major public health threat. With half the global population at risk, Paraguay experiences particularly high infection rates. Despite this, data on the seroprevalence of these viruses in this country is lacking. This study aims to assess the seroprevalence of anti-DENV IgG and anti-CHIKV IgG among blood donors in Paraguay. Serum samples from 546 blood donors across seven regional districts and Asunción were collected from March to May 2023. Participants filled out a questionnaire and underwent eligibility screening. Serum samples were tested for anti-DENV IgG and anti-CHIKV IgG antibodies using immunoassays. Data were analyzed using IBM SPSS version 23.0. The median (IQR) age of donors was 34 (26-44), and 47.1% were female. Anti-DENV IgG prevalence was 87.7%, ranging from 73.7% to 100% by location, with an age-related association. Donors aged 18 to 25 had a 79.2% seroprevalence, while those over 46 had the highest at 91.5% (p = 0.010). Anti-CHIKV IgG prevalence was 37.2%, with men showing a seroprevalence nearly 10% higher than women, but no significant age-related differences were observed. Regional variation in CHIKV seroprevalence was not significant. In conclusion, this study suggests a high seroprevalence of both DENV and CHIKV in Paraguayan blood donors. The high DENV seroprevalence reflects the impact of past outbreaks, while the notable CHIKV prevalence underscores the effects of recent outbreaks. Continuous surveillance, improved diagnostics, and effective vector control measures are essential to mitigate these arboviruses' impact in Paraguay.
登革病毒（DENV）和基孔肯雅病毒（CHIKV）等重新出现的病原体的崛起构成了重大的公共卫生威胁。全球一半人口面临风险，巴拉圭的感染率尤其高。尽管如此，该国缺乏这些病毒血清流行率的数据。本研究旨在评估巴拉圭献血者中抗DENV IgG和抗CHIKV IgG的血清流行率。2023年3月至5月，从七个地区和亚松森的546名献血者中采集了血清样本。参与者填写了问卷并接受了资格筛查。使用免疫测定法检测血清样本中的抗DENV IgG和抗CHIKV IgG抗体。使用IBM SPSS 23.0版分析数据。献血者的年龄中位数（四分位距）为34岁（26 - 44岁），47.1%为女性。抗DENV IgG流行率为87.7%，按地点范围从73.7%到100%，与年龄相关。18至25岁的献血者血清流行率为79.2%，而46岁以上的献血者最高，为91.5%（p = 0.010）。抗CHIKV IgG流行率为37.2%，男性的血清流行率比女性高近10%，但未观察到明显的年龄相关差异。CHIKV血清流行率的区域差异不显著。总之，本研究表明巴拉圭献血者中DENV和CHIKV的血清流行率都很高。高DENV血清流行率反映了过去疫情的影响，而显著的CHIKV流行率凸显了近期疫情的影响。持续监测、改进诊断和有效的病媒控制措施对于减轻这些虫媒病毒在巴拉圭的影响至关重要。
