Kuttoth Haritha, Pathak Tushar, Sandhyarani N
Nanoscience Research Laboratory, Department of Materials Science and Engineering, National Institute of Technology Calicut, Calicut 673601, Kerala, India.
Langmuir. 2025 Jul 8;41(26):16822-16831. doi: 10.1021/acs.langmuir.5c00784. Epub 2025 May 13.
The rising demand for noninvasive portable point-of-care (POC) sensors for on-site detection of biomarkers reflects a shift toward personalized healthcare and real-time diagnostics. Lipopolysaccharide (LPS) is a key sepsis biomarker, a bacteria-borne endotoxin that can induce fatal conditions, such as septic shock, if left undetected. Timely bedside detection of LPS is critical for the appropriate intervention and treatment of sepsis. In this work, we report a highly sensitive electrochemical sensor chip designed for the selective detection of LPS, which is compatible with a portable analyzer for on-site detection. The sensor is fabricated using functionalized CNT (fCNT) and copper(I) oxide nanoparticles (CuO). Functionalized with the LPS-specific aptamer, the sensor demonstrated remarkable selectivity and sensitivity toward LPS. It achieved a limit of detection (LOD) of 10 ag mL and exhibited a linear detection range from 10 ag mL to 10 ng mL. The device detected LPS across various samples, including food samples such as mayonnaise and fruit juices, as well as a clinical sample, whole blood. These results underscore its potential for practical application in food quality assurance and clinical diagnostics, offering real-time analysis capabilities.
对用于现场检测生物标志物的非侵入式便携式即时检测（POC）传感器的需求不断增加，这反映了向个性化医疗和实时诊断的转变。脂多糖（LPS）是一种关键的脓毒症生物标志物，一种细菌源性内毒素，如果未被检测到，可诱发致命状况，如脓毒性休克。及时在床边检测LPS对于脓毒症的适当干预和治疗至关重要。在这项工作中，我们报告了一种专为选择性检测LPS而设计的高灵敏度电化学传感器芯片，它与用于现场检测的便携式分析仪兼容。该传感器采用功能化碳纳米管（fCNT）和氧化铜纳米颗粒（CuO）制造。用LPS特异性适配体进行功能化后，该传感器对LPS表现出显著的选择性和灵敏度。它实现了10 ag/mL的检测限（LOD），并呈现出从10 ag/mL到10 ng/mL的线性检测范围。该设备可检测各种样品中的LPS，包括蛋黄酱和果汁等食品样品以及临床样品全血。这些结果突出了其在食品质量保证和临床诊断中实际应用的潜力，提供了实时分析能力。
