Viso M, el Jaraki M R, Espinasse J, Parodi A L, Dedieu J F
Vet Res Commun. 1985 Jul;9(3):213-9. doi: 10.1007/BF02215144.
The method of lung lavage under fiberoptic control allowed collection of alveolar cells in non-anaesthetized adult cows. The median section of the diaphragmatic lobe was lavaged with five consecutive aliquots of 30 ml each. Every one was analysed separately. A mean of 25.6% of instilled fluid was recovered and this is lower than amounts obtained on isolated lungs or in anaesthetized calves (about 50%). The cellular formula of 30 samples showed 83.5% of macrophages, 6.0% of lymphocytes, 9.4% of polymorphonuclear cells, 0.5% of monocytes. Cellular viability and total cell count were similar to previously published data. All results were found to be independent of the washing sequence. This simple and well tolerated technique appeared to be a useful tool for the study of defence mechanisms of deep lung.
在纤维光学控制下进行肺灌洗的方法能够在未麻醉的成年母牛身上采集肺泡细胞。对膈叶的中间部分用连续五份每份30毫升的液体进行灌洗。每份灌洗液均单独进行分析。回收的灌洗液平均为注入量的25.6%，这低于在离体肺或麻醉小牛身上获得的量（约50%）。30个样本的细胞组成显示，巨噬细胞占83.5%，淋巴细胞占6.0%，多形核细胞占9.4%，单核细胞占0.5%。细胞活力和总细胞计数与先前发表的数据相似。所有结果均与冲洗顺序无关。这种简单且耐受性良好的技术似乎是研究深部肺防御机制的有用工具。
