Zhuo Huasheng, He Chunhua, Yang Canfeng, Jiang Xian, Li Fan, Yang Xiangliang, Yang Hai, Yong Tuying, Liu Zhiyong, Ma Yan, Nie Lei, Liao Guanglan, Shi Tielin
State Key Laboratory of Digital Manufacturing Equipment and Technology, Huazhong University of Science and Technology, Wuhan, China.
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
Commun Eng. 2025 May 13;4(1):86. doi: 10.1038/s44172-025-00427-0.
Rare particle enrichment plays a pivotal role in advancing numerous scientific research areas and industrial processes. Traditional enrichment methods encounter obstacles such as low efficiency, high cost, and complexity. Acoustic focusing, optical fiber detection, and electrical manipulation have shown potential in microfluidics for particle manipulation and analysis. This study pioneers the integration of the acoustic, optical, and electrical units to overcome the traditional limitations. The cooperative dynamics of acoustic and flow focusing are explored. The optical fibers with an enhanced detection algorithm greatly boost optical detection sensitivity. Furthermore, the droplet charging to enhance the tip charging phenomenon is complemented and validated. The detection and sorting accuracy of enriching large-size H22 cells reached 99.8% and 99.3%, respectively, with the target cell concentration increased by nearly 86-fold. Our work significantly enhances detection sensitivity and particle manipulation accuracy, ultimately offering a robust and reliable solution for generating droplets to enrich rare particles.
稀有粒子富集在推动众多科研领域和工业流程方面发挥着关键作用。传统的富集方法面临着诸如效率低、成本高和操作复杂等障碍。声聚焦、光纤检测和电操控在微流控技术中展现出了用于粒子操控和分析的潜力。本研究率先将声学、光学和电学单元集成，以克服传统方法的局限性。探索了声聚焦与流动聚焦的协同动力学。采用增强检测算法的光纤极大地提高了光学检测灵敏度。此外，对液滴充电以增强尖端充电现象进行了补充和验证。富集大尺寸H22细胞的检测和分选准确率分别达到了99.8%和99.3%，目标细胞浓度提高了近86倍。我们的工作显著提高了检测灵敏度和粒子操控精度，最终为生成用于富集稀有粒子的液滴提供了一种强大且可靠的解决方案。
