Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, Guizhou Province, China.
School of Exercise and Health, Guangzhou Sport University, Guangzhou 510500, China.
Int J Biol Macromol. 2024 May;268(Pt 2):131910. doi: 10.1016/j.ijbiomac.2024.131910. Epub 2024 Apr 26.
In this study, polysaccharides (RRTPs) were extracted from Rosa roxburghii Tratt pomace by hot water or ultrasound (US)-assisted extraction. The structural properties and potential prebiotic functions of RRTPs were investigated. Structural characterization was conducted through HPAEC, HPGPC, GC-MS, FT-IR and SEM. Chemical composition analysis revealed that RRTPs extracted by hot water (RRTP-HW) or US with shorter (RRTP-US-S) or longer duration (RRTP-US-L) all consisted of galacturonic acid, galactose, glucose, arabinose, rhamnose and glucuronic acid in various molar ratio. US extraction caused notable reduction in molecular weight of RRTPs but no significant changes in primary structures. Fecal fermentation showed RRTPs could reshape microbial composition toward a healthier balance, leading to a higher production of beneficial metabolites including total short-chain fatty acids, curcumin, noopept, spermidine, 3-feruloylquinic acid and citrulline. More beneficial shifts in bacterial population were observed in RRTP-HW group, while RRTP-US-S had stronger ability to stimulate bacterial short-chain fatty acids production. Additionally, metabolic profiles with the intervention of RRTP-HW, RRTP-US-S or RRTP-US-L were significantly different from each other. The results suggested RRTPs had potential prebiotic effects which could be modified by power US via molecular weight degradation.
在这项研究中，从玫瑰废渣中用水热法或超声（US）辅助提取多糖（RRTPs）。研究了 RRTPs 的结构特性和潜在的益生元功能。通过 HPAEC、HPGPC、GC-MS、FT-IR 和 SEM 进行结构表征。化学成分分析表明，热水（RRTP-HW）或 US 提取的 RRTP（RRTP-US-S 或 RRTP-US-L）均由半乳糖醛酸、半乳糖、葡萄糖、阿拉伯糖、鼠李糖和葡萄糖醛酸以不同的摩尔比组成。US 提取导致 RRTPs 的分子量显著降低，但对其一级结构没有显著影响。粪便发酵表明 RRTPs 可以重塑微生物组成，使其更趋于健康平衡，从而产生更多有益代谢物，包括总短链脂肪酸、姜黄素、noopept、亚精胺、3-阿魏酰奎宁酸和瓜氨酸。RRTP-HW 组观察到细菌种群的有益变化更大，而 RRTP-US-S 具有更强的刺激细菌短链脂肪酸产生的能力。此外，RRTP-HW、RRTP-US-S 或 RRTP-US-L 干预的代谢谱彼此明显不同。结果表明，RRTPs 具有潜在的益生元作用，这种作用可以通过功率 US 进行分子量降解来修饰。
