Yu Hairui, Nazir Sadia, Ijaz Farah, Zahid Muhammad Umer, Mushtaq Maida, Khan Muhammad, Rahman Abdur, Rahman Muhammad Aziz Ur
Weifang Key Laboratory of Coho Salmon Culturing Facility Engineering, Institute of Modern Facility Fisheries, College of Biology and Oceanography, Weifang University, Weifang 261061, China.
Department of Fisheries & Aquaculture, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
Animals (Basel). 2025 Apr 29;15(9):1256. doi: 10.3390/ani15091256.
This study evaluated the impact of supplementation on growth, antioxidant status, digestive enzyme activities, hematological parameters, blood biochemistry, and immune responses in Nile tilapia () fingerlings. A total of 180 fingerlings (mean weight: 5 ± 0.5 g) were randomly assigned to four groups: a control group and three probiotic-supplemented groups with 10 (S-1), 10 (S-2), and 10 (S-3) CFU g of , following a completely randomized design. Fish were fed twice daily for eight weeks. Results showed that the 10 CFU g group exhibited significantly higher weight gain (43 g), specific growth rate (2.5%/day), and better feed conversion ratio (0.7) compared to the control (30 g, 2.2%/day, and 1.4, respectively). Hematological parameters (WBC, RBC, hemoglobin) and immune responses (phagocytic activity, lysozyme) were significantly improved. Blood biochemistry showed decreased ALT/AST levels and increased ALP activity. Digestive enzymes (protease, lipase, amylase) and antioxidant activity (GPx, CAT, SOD) were enhanced, with reduced MDA levels. No significant differences were observed in survival rates or stress responses. These findings suggest that supplementation at 10 CFU g improves growth, immune function, and antioxidant status, making it a promising probiotic for Nile tilapia.
本研究评估了添加物对尼罗罗非鱼（）幼鱼生长、抗氧化状态、消化酶活性、血液学参数、血液生化指标及免疫反应的影响。总共180尾幼鱼（平均体重：5±0.5克）被随机分为四组：一个对照组和三个添加益生菌的组，分别添加10（S-1）、10（S-2）和10（S-3）CFU/克的，采用完全随机设计。鱼每天投喂两次，持续八周。结果显示，与对照组（分别为30克、2.2%/天和1.4）相比，10CFU/克组的体重增加显著更高（43克）、特定生长率（2.5%/天）更好且饲料转化率更高（0.7）。血液学参数（白细胞、红细胞、血红蛋白）和免疫反应（吞噬活性、溶菌酶）显著改善。血液生化指标显示谷丙转氨酶/谷草转氨酶水平降低且碱性磷酸酶活性增加。消化酶（蛋白酶、脂肪酶、淀粉酶）和抗氧化活性（谷胱甘肽过氧化物酶、过氧化氢酶、超氧化物歧化酶）增强，丙二醛水平降低。在存活率或应激反应方面未观察到显著差异。这些发现表明，添加10CFU/克的可改善尼罗罗非鱼的生长、免疫功能和抗氧化状态，使其成为一种有前景的尼罗罗非鱼益生菌。
