Van Doan Hien, Wangkahart Eakapol, Thaimuangphol Wipavee, Panase Paiboon, Sutthi Nantaporn
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand.
Science and Technology Research Institute, Chiang Mai University, Chiang Mai, 50200, Thailand.
Probiotics Antimicrob Proteins. 2023 Apr;15(2):363-378. doi: 10.1007/s12602-021-09845-w. Epub 2021 Oct 1.
The purpose of this study was to evaluate the effect of Bacillus spp. mixture (Bacillus subtilis TISTR001, Bacillus megaterium TISTR067, and Bacillus licheniformis DF001) (1 × 10 CFU/g) on growth, immune parameters, immune-related gene expression, and resistance of Nile tilapia against Streptococcus agalactiae AAHM04. Fish were fed different concentrations of Bacillus spp. 0 (control; T1), 1 (T2), 3 (T3), and 5 (T4) g/kg diets for 120 days. The results showed that weight gain, average daily gain, specific growth rate, feed conversion ratio in T3 diet were significantly higher than the control group and other tested diets (p < 0.05). Immune parameters, such as myeloperoxidase and lysozyme, were significantly higher in the T3 and T4 diets compared to the control group (p < 0.05). Similarly, IL-1β and TNF-α gene expressions in the spleen of fish fed T2, T3, and T4 diets were significantly higher than the control group (p < 0.05). However, no significant differences in survival rate, hematology, blood chemical indices, malondialdehyde (MDA) levels, body chemical composition, and organosomatic indices (p > 0.05) were noticed in all treatments. No significant differences in survival rate after the challenge test with S. agalactiae AAHM04 were found in fish fed Bacillus spp. mixture diets, except for the T3 diet. These results suggest that Bacillus spp. mixture diet at 3 g/kg diet (T3) could improve growth, immune response, and disease resistance of Nile tilapia.
本研究的目的是评估芽孢杆菌属混合物（枯草芽孢杆菌TISTR001、巨大芽孢杆菌TISTR067和地衣芽孢杆菌DF001）（1×10 CFU/g）对尼罗罗非鱼生长、免疫参数、免疫相关基因表达以及抵抗无乳链球菌AAHM04能力的影响。给鱼投喂不同浓度（0（对照；T1）、1（T2）、3（T3）和5（T4）g/kg）的芽孢杆菌属混合物饲料，持续120天。结果表明，T3组饲料的增重、平均日增重、特定生长率、饲料转化率显著高于对照组和其他受试饲料组（p＜0.05）。与对照组相比，T3和T4组饲料中的免疫参数，如髓过氧化物酶和溶菌酶，显著更高（p＜0.05）。同样，投喂T2、T3和T4组饲料的鱼脾脏中IL-1β和TNF-α基因表达显著高于对照组（p＜0.05）。然而，所有处理组在存活率、血液学指标、血液生化指标、丙二醛（MDA）水平、鱼体化学成分和脏体比方面均未发现显著差异（p＞0.05）。在用无乳链球菌AAHM04进行攻毒试验后，除T3组饲料外，投喂芽孢杆菌属混合物饲料的鱼在存活率上未发现显著差异。这些结果表明，3 g/kg饲料（T3）的芽孢杆菌属混合物饲料可提高尼罗罗非鱼的生长、免疫反应和抗病能力。
