Becchimanzi Andrea, Zimowska Beata, Calandrelli Marina Maura, De Masi Luigi, Nicoletti Rosario
Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy.
Department of Plant Protection, University of Life Sciences, 20-069 Lublin, Poland.
Int J Mol Sci. 2025 May 5;26(9):4377. doi: 10.3390/ijms26094377.
This study reports on the whole genome sequencing of the hazelnut endophytic isolate Hzn5 from Poland. It was identified as a member of the species complex based on a phylogenetic analysis which also pointed out that other hazelnut isolates, previously identified as and , actually belong to this species complex. Genome annotation allowed the mapping of 4491 different protein sequences to the genome assembly. A further in silico search for their potential biosynthetic activity showed that predicted genes are involved in 1110 metabolic pathways. Moreover, the analysis of the genome sequence carried out in comparison to another isolate, previously identified as an agent of hazelnut gray necrosis in Italy, revealed a homology to several regions containing biosynthetic gene clusters for bioactive secondary metabolites. The resulting indications for the biosynthetic aptitude concerning some emerging mycotoxins, such as the enniatins and culmorin, should be taken into consideration with reference to the possible contamination of hazelnuts and derived products.
本研究报告了来自波兰的榛子内生分离株Hzn5的全基因组测序情况。基于系统发育分析，它被鉴定为该物种复合体的一个成员，该分析还指出，其他先前被鉴定为[具体名称1]和[具体名称2]的榛子分离株实际上也属于这个物种复合体。基因组注释使得4491种不同的蛋白质序列能够映射到基因组组装上。进一步对其潜在生物合成活性进行的计算机模拟搜索表明，预测基因参与了1110条代谢途径。此外，与另一个先前被鉴定为意大利榛子灰坏死病原体的分离株相比，对该基因组序列的分析揭示了与几个含有生物活性次生代谢物生物合成基因簇的区域具有同源性。关于一些新兴霉菌毒素（如恩镰孢菌素和枯菌素）的生物合成能力的结果表明，应考虑到榛子及其衍生产品可能受到的污染。
