He Xiaofang, Ma Longfei, Zhang Jiaqi, Zhou Binbin, Chen Shun, Tu Minhang, Cai Gentan, Wang Tian, Wang Chao
College of Animal Science and Food Engineering, Jinling Institute of Science and Technology, Nanjing 211169, China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Nutrients. 2025 Apr 29;17(9):1495. doi: 10.3390/nu17091495.
In animal production, nanoparticulate zinc oxide exhibits synergistic antibacterial efficacy coupled with growth-promoting effects, positioning itself as a novel antibiotic alternative with enhanced biosafety profiles. However, its dose-dependent toxicity poses challenges. The experimental design sought to quantify the protective effects of dietary rutin against zinc-overload-induced damage. A zinc-overload murine model was established by giving high-dose ZnO nanoparticles (HZn, 5000 mg/kg/day) for 21 days. Mice were then fed rutin at doses of 300, 600, or 1200 mg/kg. Body weight, relative organ indexes, zinc concentrations, serum enzyme activities, and tissue-level indicators of apoptosis, autophagy, mitochondrial function, and antioxidant capacity were measured. The results showed that rutin could not reverse HZn-induced body weight decline but improved relative organ indexes in liver and kidney. It alleviated HZn-induced cell damage and enhanced antioxidant capacity in jejunum and serum through activation, without inhibiting HZn-induced zinc elevation. Rutin, especially at 600 mg/kg, can partially restore hepatic function and organ index and mitigate HZn-induced hepatic and jejunal injuries.
在动物生产中，纳米氧化锌表现出协同抗菌功效以及促生长作用，使其成为一种具有更高生物安全性的新型抗生素替代品。然而，其剂量依赖性毒性带来了挑战。本实验设计旨在量化日粮芦丁对锌过载诱导损伤的保护作用。通过给予高剂量氧化锌纳米颗粒（HZn，5000毫克/千克/天）21天建立锌过载小鼠模型。然后给小鼠喂食剂量为300、600或1200毫克/千克的芦丁。测量体重、相对器官指数、锌浓度、血清酶活性以及细胞凋亡、自噬、线粒体功能和抗氧化能力的组织水平指标。结果表明，芦丁不能逆转HZn诱导的体重下降，但改善了肝脏和肾脏的相对器官指数。它通过激活减轻了HZn诱导的细胞损伤，并增强了空肠和血清中的抗氧化能力，而没有抑制HZn诱导的锌升高。芦丁，尤其是600毫克/千克时，可部分恢复肝功能和器官指数，并减轻HZn诱导的肝脏和空肠损伤。
