Skorupa Agata, Jakubczyk Magdalena, Michalkiewicz Slawomir
Institute of Chemistry, Jan Kochanowski University, 7 Uniwersytecka St., PL-25406 Kielce, Poland.
Materials (Basel). 2025 Apr 29;18(9):2044. doi: 10.3390/ma18092044.
In this study, the voltammetric behavior of apocynin on a boron-doped diamond electrode in a phosphate buffer (pH 7.3) has been reported for the first time. The oxidation process is quasi-reversible, diffusion-controlled, and involves one electron and one proton. The product of the electrode reaction is an unstable radical that undergoes successive chemical transformations near the working electrode. The proposed mechanism of this process can be described as and served as the basis for the development of a new voltammetric method for determining apocynin in natural samples. The analytical signal was the anodic peak on DPV curves at a potential of 0.605 V vs. Ag/AgCl. A linear response was observed in the concentration range of 0.213-27.08 mg L. The estimated and values were 0.071 and 0.213 mg L, respectively. The effectiveness of the method was demonstrated both in control determinations and in the analysis of the dietary supplement. This procedure is simple, fast, sensitive, selective, and requires no complicated sample preparation, which is limited only to a simple extraction with ethanol. The low consumption of non-toxic reagents makes it environmentally friendly. To the best of our knowledge, this is the first presentation of a voltammetric procedure to determine this analyte studied in a phosphate buffer solution on a boron-doped diamond electrode. It can also be easily adapted to determine other phenolic compounds with antioxidant properties in various matrices.
本研究首次报道了阿扑辛在硼掺杂金刚石电极上于磷酸盐缓冲液（pH 7.3）中的伏安行为。氧化过程为准可逆、扩散控制，涉及一个电子和一个质子。电极反应产物是一个不稳定的自由基，在工作电极附近发生连续的化学转化。该过程的 proposed mechanism 可描述为，并作为开发一种测定天然样品中阿扑辛的新伏安法的基础。分析信号是差分脉冲伏安曲线（DPV）上相对于Ag/AgCl在0.605 V电位处的阳极峰。在0.213 - 27.08 mg/L的浓度范围内观察到线性响应。估计的 和 值分别为0.071和0.213 mg/L。该方法在对照测定和膳食补充剂分析中均得到了验证。该程序简单、快速、灵敏、选择性好，无需复杂的样品制备，仅需用乙醇进行简单萃取。无毒试剂消耗低，使其对环境友好。据我们所知，这是首次在硼掺杂金刚石电极上于磷酸盐缓冲溶液中测定该分析物的伏安法。它也可轻松适用于测定各种基质中具有抗氧化特性的其他酚类化合物。 （注：原文中“proposed mechanism”未翻译完整，可能存在信息缺失）
