Li Yihua, Li Junmei, Cai Xiaoxia, Gao Meiyu, Diao Hongliang, Xiang Huiming, Zhou Wenwen, Ma Ruiyan
College of Plant Protection, Shanxi Agricultural University, Jinzhong, China.
Front Microbiol. 2025 Apr 29;16:1583850. doi: 10.3389/fmicb.2025.1583850. eCollection 2025.
IF-1106 is an entomopathogenic fungus with a wide range of hosts. It produces microsclerotia in liquid media.
This study took a close observation of the microsclerotia formation process of IF-1106 and described the surface characteristics of IF-1106 microsclerotia. Secondly, the viability of microsclerotia under high temperature and ultraviolet radiation were determined. Thirdly, the microsclerotia were stored under different temperatures to assess storage stability. Finally, activity of microsclerotia against root-knot nematode and the influence on the plant growth of cucumber were investigated.
Spores germinate quickly, and hyphal elements clump together, forming hydrophobic microsclerotia. The microsclerotia have 100% survival under 55°C and UV-B radiation of 3 J cm. Following such exposure, the microsclerotia are able to germinate and produce conidia. After 1 year of storage at either room temperature (25°C) or refrigeration (-80°C, -20°C, and 4°C), all the microsclerotia germinated and spore yield was more than 4 × 10 spores g. Finally, IF-1106 microsclerotia showed ovicidal activity against root-knot nematode eggs, and a glasshouse pot trial indicated that control efficiency of microsclerotia against root-knot nematodes reached 92.6%, which improved the growth of the test cucumber plants. These attributes suggest that microsclerotia of this fungus can be applied as a biocontrol agent to control soil borne pest nematodes.
IF-1106是一种宿主范围广泛的昆虫病原真菌。它能在液体培养基中产生微菌核。
本研究密切观察了IF-1106微菌核的形成过程，并描述了IF-1106微菌核的表面特征。其次，测定了微菌核在高温和紫外线辐射下的活力。第三，将微菌核在不同温度下储存以评估储存稳定性。最后，研究了微菌核对根结线虫的活性以及对黄瓜植株生长的影响。
孢子萌发迅速，菌丝体聚集在一起，形成疏水的微菌核。微菌核在55°C和3 J/cm的UV-B辐射下存活率为100%。经过这样的处理后，微菌核能够萌发并产生分生孢子。在室温（25°C）或冷藏（-80°C、-20°C和4°C）下储存1年后，所有微菌核均能萌发，孢子产量超过4×10个孢子/克。最后，IF-1106微菌核对根结线虫卵具有杀卵活性，温室盆栽试验表明微菌核对根结线虫的防治效果达到92.6%，促进了试验黄瓜植株的生长。这些特性表明，这种真菌的微菌核可作为一种生物防治剂来防治土传害虫线虫。
