一种用于次氯酸的新型荧光近红外关闭型探针及其应用。
A new turn off fluorescent NIR probe for hypochlorous acid and its applications.
作者信息
Ibrahimova Ayten, Önem Ayşe Nur, Altun Mehmet, Yeşil Emin Ahmet, Baysal Aslı, Saygin Hasan, Soyocak Ahu, Özyürek Mustafa
机构信息
Division of Analytical Chemistry, Department of Chemistry, Faculty of Engineering, Istanbul University-Cerrahpaşa, İstanbul, Turkiye.
Division of Organic Chemistry, Department of Chemistry, Faculty of Engineering, Istanbul University-Cerrahpaşa, İstanbul, Turkiye.
出版信息
Turk J Chem. 2025 Mar 29;49(2):241-253. doi: 10.55730/1300-0527.3725. eCollection 2025.
Hypochlorous acid (HOCl) is a potent nonradical oxidant involved in various physiological processes, particularly within the human immune system. In this study, we introduce a novel, rapid, and highly efficient fluorometric method for the detection of HOCl. The method utilizes a near-infrared (NIR)-based fluorescent probe, NIR-QBH, which is characterized by its high sensitivity and chemical stability. NIR-QBH, containing olefinic C=C bonds, exhibits strong NIR emission at 660 nm (λ = 618 nm). The detection mechanism relies on the oxidation of the C=C bond in the NIR-QBH structure by HOCl, resulting in the formation of non-fluorescent products. With a detection limit of 0.23 μM, the probe demonstrates a fast response time of 4 min. Glutathione (GSH), an essential biothiol, was employed as a reference HOCl scavenger, and its HOCl scavenging activity was evaluated with an IC value of 8.97 μM. Furthermore, the developed fluorometric assay was successfully applied for the detection of HOCl in fetal bovine serum (FBS) and aqueous solutions.
次氯酸（HOCl）是一种强效的非自由基氧化剂，参与各种生理过程，尤其是在人体免疫系统中。在本研究中，我们介绍了一种用于检测HOCl的新型、快速且高效的荧光测定法。该方法利用了一种基于近红外（NIR）的荧光探针NIR-QBH，其特点是具有高灵敏度和化学稳定性。NIR-QBH含有烯键式C=C键，在660 nm处呈现强近红外发射（λ = 618 nm）。检测机制依赖于HOCl对NIR-QBH结构中C=C键的氧化，导致形成非荧光产物。该探针的检测限为0.23 μM，响应时间快，为4分钟。谷胱甘肽（GSH）是一种必需的生物硫醇，用作参考HOCl清除剂，并评估其HOCl清除活性，IC值为8.97 μM。此外，所开发的荧光测定法成功应用于检测胎牛血清（FBS）和水溶液中的HOCl。
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