Wang Yueqiang, Sun Shengwei
Faculty of Science, National University of Singapore, Block S16 Level 9, 6 Science Drive 2, Singapore, 117546, Singapore.
School of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Fibre and Polymer Technology, KTH Royal Institute of Technology, 100 44, Stockholm, Sweden.
Anal Bioanal Chem. 2025 May 14. doi: 10.1007/s00216-025-05904-1.
Tetracycline antibiotics (TCs) are widely used in medicine, agriculture, and animal husbandry. However, their overuse has led to environmental pollution, posing a significant threat to water sources, soil, and food safety. Therefore, there is an urgent need for efficient, sensitive, simple, and low-cost detection methods for environmental pollution monitoring. In this study, the catalytic activity of copper-based nanozymes was regulated by AAs. Lysine, aspartic acid, glycine, and arginine were chosen as ligands to synthesize different copper-based nanozymes. The results showed that the type of amino acid significantly influenced the particle size, morphology, and peroxidase (POD)-like catalytic activity of Cu₂O. Based on these amino acid-regulated Cu₂O nanozymes, we further developed a highly sensitive, easy-to-use, and low-cost colorimetric sensor array that can effectively distinguish TCs. This sensor array was successfully validated in binary mixtures and wastewater environments. This study not only provides important insights into the small-molecule regulation of copper-based nanozyme catalytic performance but also offers a novel approach for the detection of TCs in environmental monitoring.
四环素类抗生素（TCs）在医学、农业和畜牧业中广泛使用。然而，它们的过度使用导致了环境污染，对水源、土壤和食品安全构成了重大威胁。因此，迫切需要高效、灵敏、简单且低成本的检测方法用于环境污染监测。在本研究中，氨基酸对铜基纳米酶的催化活性进行了调控。选择赖氨酸、天冬氨酸、甘氨酸和精氨酸作为配体来合成不同的铜基纳米酶。结果表明，氨基酸的种类显著影响了Cu₂O的粒径、形态和类过氧化物酶（POD）催化活性。基于这些氨基酸调控的Cu₂O纳米酶，我们进一步开发了一种高灵敏度、易于使用且低成本的比色传感器阵列，该阵列能够有效区分四环素类抗生素。该传感器阵列在二元混合物和废水环境中成功得到验证。本研究不仅为铜基纳米酶催化性能的小分子调控提供了重要见解，也为环境监测中四环素类抗生素的检测提供了一种新方法。
