Mazur L
Acta Physiol Hung. 1985;66(1):65-8.
On the first day of gestation, Porton mice were injected intraperitoneally with AET (2-aminoethylisothiouronium bromide hydrobromide), MEA (cysteamine hydrochloride,) or 5-HT (serotonin-creatinine sulphate), in a dose of 40 mg/kg of bodyweight. On the nineteenth day of pregnancy, the fresh weight of both heart and kidneys of foetuses, as well as DNA content in 25 mg of fresh tissue and in these whole organs were analysed. DNA was extracted from the foetal organs by means of Burton's method, which is based on the estimation of deoxiribose content in the colour reaction with diphenylamine. As compared to controls, in the remaining groups of mice lower fresh weight of both heart and kidneys of foetuses, greater DNA content in 25 mg of fresh tissue and smaller total amounts of DNA in the whole organs were found. Among the experimental groups of mice, statistically significant differences in the analysed values were observed between the group of animals treated with 5-HT and the remaining groups, with the exception of statistically non-significant difference in the DNA content of the whole kidneys between those injected with 5-HT and MEA.
在妊娠第一天，给波顿小鼠腹腔注射40毫克/千克体重的AET（氢溴酸2-氨基乙基异硫脲）、MEA（盐酸半胱胺）或5-HT（5-羟色胺-硫酸肌酸酐）。在妊娠第19天，分析胎儿心脏和肾脏的鲜重，以及25毫克新鲜组织和这些整个器官中的DNA含量。采用伯顿法从胎儿器官中提取DNA，该方法基于在与二苯胺的显色反应中对脱氧核糖含量的估算。与对照组相比，在其余几组小鼠中，发现胎儿心脏和肾脏的鲜重较低，25毫克新鲜组织中的DNA含量较高，而整个器官中的DNA总量较小。在小鼠实验组中，除了注射5-HT和MEA的小鼠全肾DNA含量无统计学显著差异外，在注射5-HT的动物组与其余组之间观察到分析值存在统计学显著差异。
