Faculdade de Ciências Biológicas e da Saúde, Universidade do Estado do Rio de Janeiro, Campus Zona Oeste (UERJ-ZO), Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
Proteomics Clin Appl. 2023 Sep;17(5):e2200098. doi: 10.1002/prca.202200098. Epub 2023 Feb 17.
In this work, we identified human and bacterial proteomes in the saliva from volunteers with gingivitis or healthy.
The reported population consisted of 18 volunteers (six with gingivitis and 12 healthy controls). Proteomics characterization was performed using a quantitative mass spectrometry method.
A total of 74 human and 116 bacterial proteins were identified in saliva. The major functional category that was modified in the human proteome was the immune response, followed by transport and protease inhibition. In the bacterial proteome, most of the proteins identified were from the Fusobacteria phylum, followed by Chlamydiae and Spirochaetes.
We observed statistically relevant differences in the data between the groups. The 15 most important human proteins affecting the variation between case and control groups included cystatin S, alpha amylase, lactotransferrin, and negative elongation factor E. We found that bacterial proteins from Porphyromonas gingivalis and Fusobacterium nucleatum subsp. nucleatum related to the red and orange complexes were closely correlated with the occurrence of periodontal diseases.
在这项工作中，我们鉴定了患有牙龈炎或健康志愿者唾液中的人类和细菌蛋白质组。
报告人群包括 18 名志愿者（牙龈炎 6 名，健康对照 12 名）。采用定量质谱法进行蛋白质组学特征分析。
在唾液中鉴定出了 74 个人类和 116 个细菌蛋白。人类蛋白质组中改变最大的主要功能类别是免疫反应，其次是运输和蛋白酶抑制。在细菌蛋白质组中，大多数鉴定出的蛋白质来自梭杆菌门，其次是衣原体和螺旋体。
我们观察到两组之间的数据存在统计学上的显著差异。影响病例组和对照组之间变异的 15 个人类最重要的蛋白质包括半胱氨酸蛋白酶抑制剂 S、α-淀粉酶、乳转铁蛋白和负延伸因子 E。我们发现与牙周病发生密切相关的牙龈卟啉单胞菌和核梭杆菌亚种的细菌蛋白与红色和橙色复合物有关。
