Zahran Mona, Alaryani Fatima S, El Bohi Khlood M, Abu Zeid Ehsan H, Khairy Mohamed H, Albalawi Aishah E, Alhasani Reem H, Felemban Shatha G, Korany Reda
Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Department of Biological Sciences, University of Jeddah, College of Science, Jeddah, Saudi Arabia.
PLoS One. 2025 May 14;20(5):e0322363. doi: 10.1371/journal.pone.0322363. eCollection 2025.
This study aimed to assess the protective effects of Lagenaria siceraria seed oil (LSO) on fifty male Albino rats subjected to furan exposure. Furan (FU) is a small, heterocyclic compound present in the volatile fraction of various thermally processed foods and beverages. Rats were categorized into five groups, each comprising ten rats. Group 1 served as the control group, receiving corn oil. Group 2 received LSO (3 g/kg body weight orally) for 28 days. Rats in Group 3 (FU-exposed group) received an oral administration of FU at a dosage of 16 mg/kg body weight each day for 28 days. Rats in Group 4 (Therapeutic co-treated group) were administered both LSO and subsequent FU exposure according to the previously outlined dosage regimen for 28 days. Rats in Group 5 (Protective co-treated group) received LSO seed oil for 14 days as protection then received Fu at the same mentioned doses of Fu until the end of experiment. Rats administered FU and/or LSO gained noticeably more weight than the control group. LSO significantly decreased AST and LDH levels in both the protection and treatment groups as compared to the FU-only group. It also assisted in restoring testosterone and luteinizing hormone (LH) levels that were decreased by FU, especially in the protected group. LSO also reduced kidney damage markers and normalized biomarker levels when administered with FU. The LSO-only group demonstrated normal immune response markers, similar to the control group. By changing MDA levels and increasing SOD, GSH, and TAC levels, co-treatment with LSO enhanced liver health. The control and LSO groups displayed normal spleen structure, whereas the LSO/FU group had normal seminiferous tubules with mild edema and congestion. Overall, LSO demonstrated protective and therapeutic benefits against FU-induced damage in rats.
本研究旨在评估葫芦种子油（LSO）对五十只接受呋喃暴露的雄性白化大鼠的保护作用。呋喃（FU）是一种存在于各种热处理食品和饮料挥发性成分中的小型杂环化合物。大鼠被分为五组，每组十只。第一组作为对照组，接受玉米油。第二组连续28天口服LSO（3克/千克体重）。第三组（呋喃暴露组）大鼠每天口服16毫克/千克体重的呋喃，持续28天。第四组（治疗联合处理组）按照先前概述的剂量方案同时给予LSO和随后的呋喃暴露，持续28天。第五组（保护联合处理组）先接受14天的LSO籽油作为保护，然后以相同的呋喃剂量接受呋喃直至实验结束。给予呋喃和/或LSO的大鼠体重明显比对照组增加更多。与仅接受呋喃的组相比，LSO在保护组和治疗组中均显著降低了AST和LDH水平。它还有助于恢复因呋喃而降低的睾酮和促黄体生成素（LH）水平，尤其是在保护组中。当与呋喃一起给药时，LSO还降低了肾脏损伤标志物并使生物标志物水平正常化。仅LSO组显示出与对照组相似的正常免疫反应标志物。通过改变丙二醛水平并提高超氧化物歧化酶、谷胱甘肽和总抗氧化能力水平，与LSO联合处理可增强肝脏健康。对照组和LSO组脾脏结构正常，而LSO/FU组生精小管正常，但有轻度水肿和充血。总体而言，LSO对大鼠因呋喃诱导的损伤具有保护和治疗作用。
