种子油对实验性四氯化碳诱导毒性的肝保护作用。
Hepatoprotective efficacy of seeds oil against experimentally carbon tetrachloride-induced toxicity.
机构信息
Clinical Nutrition Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi Arabia.
College of Pharmacy, Al-Mustaqbal University, Babylon, Iraq.
出版信息
Open Vet J. 2024 Aug;14(8):2016-2028. doi: 10.5455/OVJ.2024.v14.i8.31. Epub 2024 Aug 31.
BACKGROUND
The liver is crucial for maintaining normal metabolism in the body. Various substances, such as toxic chemicals, drugs, and alcohol, can damage hepatocyte cells, leading to metabolic imbalances.
AIM
The experiment aimed to determine the efficacy of seed oil (LSS) as a hepatoprotective agent against acute hepatotoxicity triggered by carbon tetrachloride (CCl).
METHODS
A total of 20 rats were randomly separated into four groups. The control group: rats received 2 ml of distilled water orally, followed by 1.25 ml of olive oil intraperitoneally (i.p.) after 30 minutes. CCL group: rats were given a single intraperitoneal dose of 1.25 ml/kg b.w. of CCl in a 1:1 mixture with olive oil. Silymarin group: received 100 mg of silymarin per kg of b.w. diluted in 2 ml of distilled water orally, followed by CCl treatment after 30 minutes. LSS oil group: received LSS oil at 3g/kg b.w. orally, followed by CCl treatment after 30 minutes. Blood samples were collected to assess liver enzymes (AST, ALT, and ALP), proteins and bilirubin fractions, and redox status (catalase, reduced glutathione (GSH), and malondialdehyde (MDA)) were assessed in hepatic tissues. Changes in liver histopathological examination were also evaluated.
RESULTS
In CCl4-treated rats, there was a significant increase in serum liver marker enzyme activity (ALP, AST, and ALT) along with a significant elevation ( < 0.05) in total bilirubin, indirect bilirubin, and direct bilirubin compared to the control rats. However, all these parameters decreased in the CCl+ Silymarin and CCl+LSS groups compared to CCl-treated rats. There was a significant decline in total protein level and serum albumin in all experimental groups compared to the control, while globulin levels significantly increased in all experimental groups. There was a significant ( < 0.05) reduction in the level of GSH and catalase, with an increase in MDA level in CCl rats compared to other rats. Histopathological investigation of the LSS-treated group showed a hepatoprotective effect against CCl.
CONCLUSION
The study revealed that LSS oil has antioxidant activity against CCl-induced toxicity.
背景
肝脏对于维持人体正常代谢至关重要。各种物质，如有毒化学物质、药物和酒精，会损害肝细胞，导致代谢失衡。
目的
本实验旨在研究芝麻籽油（LSS）对四氯化碳（CCl）诱导的急性肝毒性的保护作用。
方法
将 20 只大鼠随机分为四组。对照组：大鼠口服给予 2ml 蒸馏水，30 分钟后腹腔内注射 1.25ml 橄榄油。CCl 组：大鼠给予 1.25ml/kg b.w.的 CCl 与橄榄油 1:1 混合液单次腹腔注射。水飞蓟素组：给予 100mg/kg b.w.的水飞蓟素溶于 2ml 蒸馏水中，口服，30 分钟后给予 CCl 处理。LSS 油组：给予 3g/kg b.w.的 LSS 油，口服，30 分钟后给予 CCl 处理。采集血样，评估肝酶（AST、ALT 和 ALP）、蛋白质和胆红素组分，评估肝组织的氧化还原状态（过氧化氢酶、还原型谷胱甘肽（GSH）和丙二醛（MDA））。还评估了肝组织病理变化。
结果
在 CCl4 处理的大鼠中，与对照组大鼠相比，血清肝标志物酶活性（ALP、AST 和 ALT）显著升高，总胆红素、间接胆红素和直接胆红素显著升高（<0.05）。然而，与 CCl 处理的大鼠相比，在 CCl+水飞蓟素和 CCl+LSS 组中，所有这些参数均降低。与对照组相比，所有实验组的总蛋白水平和血清白蛋白水平均显著降低，而所有实验组的球蛋白水平均显著升高。与其他大鼠相比，CCl 大鼠的 GSH 和过氧化氢酶水平显著降低，MDA 水平显著升高。LSS 处理组的组织病理学检查显示其对 CCl 具有保护作用。
结论
本研究表明，LSS 油具有对抗 CCl 诱导的毒性的抗氧化活性。
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