Kim Jungeun, Park Kiyeon, Hu Houhua, Lee Chanhee
Interdisciplinary Nutrition Program, Department of Animal Sciences, The Ohio State University, Wooster, OH, USA.
Department of Animal Sciences, The Ohio State University, Wooster, OH, USA.
Anim Biosci. 2025 Oct;38(10):2149-2154. doi: 10.5713/ab.25.0130. Epub 2025 May 12.
The current study was part of large experiments in a replicated 4×4 Latin square design where 4 dietary treatments (feed supplements) were examined. Fecal samples were obtained from the experiments via total collection (Experiment 1, n = 30; Experiment 2, n = 16). Feed and fecal samples were analyzed for acid detergent insoluble ash (ADIA) and nutrients, and those were used to estimate fecal output (FO) and nutrient digestibilites. Data within each experiment were analyzed using the Mixed procedure of SAS to determine diet, method (actual vs. estimated by ADIA), and their interaction on FO and apparent total tract digestibility of nutrients. A linear regression for FO between actual and estimated by ADIA was also conducted. The recovery of ADIA was 106% in both experiments. The use of ADIA tended to underestimate FO by 5% (Exp. 2, p = 0.09) and overestimate nutrient digestibilities by 2% to 5% (Exp. 1, p≤0.08). The linear regression resulted in the slope of 0.80 (against 1, p = 0.02) and the intercept of 1.93 (against 0, p = 0.21). In conclusion, ADIA is a potential marker for lactating cows in estimating FO when corrected by the factor obtained from the regression analysis.
本研究是一项大型实验的一部分，该实验采用重复的4×4拉丁方设计，对4种日粮处理（饲料添加剂）进行了研究。通过全收粪法从实验中获取粪便样本（实验1，n = 30；实验2，n = 16）。对饲料和粪便样本进行酸性洗涤不溶灰分（ADIA）和养分分析，并用于估计粪便排出量（FO）和养分消化率。每个实验中的数据使用SAS的混合程序进行分析，以确定日粮、方法（实际值与ADIA估计值）及其对FO和养分表观全肠道消化率的交互作用。还对ADIA估计值与实际值之间的FO进行了线性回归分析。两个实验中ADIA的回收率均为106%。使用ADIA往往会使FO低估5%（实验2，p = 0.09），使养分消化率高估2%至5%（实验1，p≤0.08）。线性回归得出斜率为0.80（相对于1，p = 0.02），截距为1.93（相对于0，p = 0.21）。总之，当通过回归分析获得的因子进行校正时，ADIA是估计泌乳奶牛FO的一个潜在指标。
