Ge Qidong, Zhang Wei, Li Chao, Li Xinlin, Wang Zhen, Li Xujun
Department of Oncology, Ningbo No. 2 Hospital, Ningbo, Zhejiang, People's Republic of China.
Department of Breast Surgery, Ningbo No. 2 Hospital, Ningbo, Zhejiang, People's Republic of China.
J Cell Mol Med. 2025 May;29(10):e70602. doi: 10.1111/jcmm.70602.
Breast cancer significantly affects women's lives globally. While PAX7 (Paired Box 7), a regulatory protein linked to muscle growth, has been connected to various cancers, its role in breast cancer is not well understood. This study explores PAX7's significance in breast cancer and its mechanisms. RNA-seq data from the TCGA database assessed PAX7 expression across cancer types. Prognostic value in breast cancer was evaluated using Kaplan-Meier and Cox regression analyses. Functional experiments, including high-throughput sequencing, cell growth analysis, colony formation, Transwell assays, and Western blot analysis, were conducted on PAX7 knockdown cell lines (MDA-MB-468 and MDA-MB-231). Results showed high PAX7 expression in breast cancer linked to lower survival rates. PAX7 knockdown affected over 2000 genes and inhibited cancer cell proliferation, migration, and invasion, involving the Wnt/β-catenin pathway. SKL2001 reversed these effects. PAX7 is a potential prognostic biomarker and therapeutic target, with elevated levels indicating a poor prognosis. Further research on PAX7-targeted therapies is needed.
乳腺癌在全球范围内对女性的生活产生重大影响。虽然PAX7（配对盒7）是一种与肌肉生长相关的调节蛋白，已被发现与多种癌症有关，但其在乳腺癌中的作用尚不清楚。本研究探讨了PAX7在乳腺癌中的意义及其作用机制。利用来自TCGA数据库的RNA测序数据评估了PAX7在不同癌症类型中的表达情况。采用Kaplan-Meier法和Cox回归分析评估PAX7在乳腺癌中的预后价值。对PAX7基因敲低的细胞系（MDA-MB-468和MDA-MB-231）进行了功能实验，包括高通量测序、细胞生长分析、集落形成实验、Transwell实验和蛋白质免疫印迹分析。结果显示，乳腺癌中PAX7高表达与较低的生存率相关。PAX7基因敲低影响了2000多个基因，并抑制了癌细胞的增殖、迁移和侵袭，这一过程涉及Wnt/β-连环蛋白信号通路。SKL2001可逆转这些效应。PAX7是一种潜在的预后生物标志物和治疗靶点，其水平升高表明预后不良。需要对PAX7靶向治疗进行进一步研究。
