Koto Serina, Arima Kazuhiko, Nakashima Hiroki, Takatani Ayuko, Mizukami Satoshi, Shimizu Yuji, Tamai Mami, Kawakami Atsushi, Hamada Koichiro, Maeda Takahiro, Kawashiri Shin-Ya, Nagata Yasuhiro, Aoyagi Kiyoshi
Department of Public Health, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JPN.
Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JPN.
Cureus. 2025 Apr 13;17(4):e82193. doi: 10.7759/cureus.82193. eCollection 2025 Apr.
Aim We aimed to investigate the association between single nucleotide polymorphisms (SNPs) in the angiotensin-converting enzyme (ACE) gene and hypertension in elderly Japanese individuals. Methods This cross-sectional validation study was nested within the Nagasaki Islands Study (NaIS), involving 1,766 community-dwelling participants aged 65 years and older. Height and weight were measured. Body mass index (BMI) was calculated from height and weight, and blood pressure was measured by trained staff. Antihypertensive medication use and lifestyle factors, including alcohol consumption, smoking habits, and exercise, were assessed by interviews. The SNP (rs4309) was genotyped using fluorescent hydrolysis probes from peripheral blood mononuclear cells. Hypertension was defined as using antihypertensive medication or a systolic blood pressure of ≥140 mmHg and/or a diastolic blood pressure of ≥90 mmHg in those not on medication. Logistic regression analysis was used to calculate adjusted odds ratios (ORs) for hypertension, adjusting for potential confounders. Results Hypertension prevalence was higher in the CC/CT genotype group compared to the TT group (71.5% vs 66.4%, p=0.025). Logistic regression showed that the CC/CT genotype was independently associated with a higher likelihood of hypertension than the TT genotype (OR=1.25, 95% confidence interval (CI) 1.001-1.548) after adjusting for age, BMI, gender, alcohol consumption, smoking, and exercise. Conclusion The CC and CT genotypes of the ACE gene were independently associated with hypertension, regardless of age, BMI, gender, and lifestyle factors. These findings support a genetically informed approach to hypertension prevention.
目的 我们旨在研究血管紧张素转换酶（ACE）基因中的单核苷酸多态性（SNP）与日本老年个体高血压之间的关联。方法 这项横断面验证研究嵌套于长崎岛研究（NaIS）中，纳入了1766名65岁及以上的社区居住参与者。测量身高和体重。根据身高和体重计算体重指数（BMI），并由经过培训的工作人员测量血压。通过访谈评估抗高血压药物使用情况以及包括饮酒、吸烟习惯和运动在内的生活方式因素。使用外周血单个核细胞的荧光水解探针进行SNP（rs4309）基因分型。高血压的定义为正在使用抗高血压药物，或未使用药物者收缩压≥140 mmHg和/或舒张压≥90 mmHg。采用逻辑回归分析计算高血压的调整优势比（OR），并对潜在混杂因素进行校正。结果 与TT基因型组相比，CC/CT基因型组的高血压患病率更高（71.5%对66.4%，p = 0.025）。逻辑回归显示，在调整年龄、BMI、性别、饮酒、吸烟和运动因素后，CC/CT基因型与高血压发生可能性较高独立相关，相比TT基因型（OR = 1.25，95%置信区间（CI）1.001 - 1.548）。结论 ACE基因的CC和CT基因型与高血压独立相关，不受年龄、BMI、性别和生活方式因素影响。这些发现支持采用基于遗传信息的高血压预防方法。
