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Science. 2023 Sep;381(6661):eadh5207. doi: 10.1126/science.adh5207. Epub 2023 Sep 1.
2
Insulin dysregulation drives mitochondrial cholesterol metabolite accumulation: initiating hepatic toxicity in nonalcoholic fatty liver disease.胰岛素失调导致线粒体胆固醇代谢产物积累：在非酒精性脂肪性肝病中引发肝毒性。
J Lipid Res. 2023 May;64(5):100363. doi: 10.1016/j.jlr.2023.100363. Epub 2023 Mar 24.
3
Regulation of cholesterol homeostasis in health and diseases: from mechanisms to targeted therapeutics.胆固醇稳态在健康和疾病中的调节：从机制到靶向治疗。
Signal Transduct Target Ther. 2022 Aug 2;7(1):265. doi: 10.1038/s41392-022-01125-5.
4
Atherosclerosis: Recent developments.动脉粥样硬化：最新进展。
Cell. 2022 May 12;185(10):1630-1645. doi: 10.1016/j.cell.2022.04.004. Epub 2022 May 2.
5
Reactive oxygen species (ROS)-responsive size-reducible nanoassemblies for deeper atherosclerotic plaque penetration and enhanced macrophage-targeted drug delivery.用于更深层动脉粥样硬化斑块渗透和增强巨噬细胞靶向药物递送的活性氧（ROS）响应性可减小尺寸的纳米组装体。
Bioact Mater. 2022 Apr 7;19:115-126. doi: 10.1016/j.bioactmat.2022.03.041. eCollection 2023 Jan.
6
Macrophage-targeted nanomedicine for the diagnosis and treatment of atherosclerosis.载脂蛋白靶向纳米医学用于动脉粥样硬化的诊断和治疗。
Nat Rev Cardiol. 2022 Apr;19(4):228-249. doi: 10.1038/s41569-021-00629-x. Epub 2021 Nov 10.
7
The changing landscape of atherosclerosis.动脉粥样硬化的变化格局。
Nature. 2021 Apr;592(7855):524-533. doi: 10.1038/s41586-021-03392-8. Epub 2021 Apr 21.
8
Association Between Periodontal Disease and Atherosclerotic Cardiovascular Diseases: Revisited.牙周病与动脉粥样硬化性心血管疾病之间的关联：再探讨
Front Cardiovasc Med. 2021 Jan 15;7:625579. doi: 10.3389/fcvm.2020.625579. eCollection 2020.
9
Macrophage membrane functionalized biomimetic nanoparticles for targeted anti-atherosclerosis applications.巨噬细胞膜功能化仿生纳米颗粒用于靶向抗动脉粥样硬化应用。
Theranostics. 2021 Jan 1;11(1):164-180. doi: 10.7150/thno.47841. eCollection 2021.
10
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Biomaterials. 2020 Nov;260:120333. doi: 10.1016/j.biomaterials.2020.120333. Epub 2020 Aug 15.

通过使用半乳糖功能化树枝状纳米颗粒进行CEH基因递送增强胆固醇流出和动脉粥样硬化消退

Enhanced Cholesterol Efflux and Atherosclerosis Regression via CEH Gene Delivery Using Galactose-Functionalized Dendrimeric Nanoparticles.

作者信息

Kou Huari, Wang Jing, Yannie Paul J, Huang Da, Korzun William J, Kakiyama Genta, Ghosh Siddhartha S, Yang Hu

机构信息

Linda and Bipin Doshi Department of Chemical and Biochemical Engineering, Missouri University of Science and Technology, Rolla, Missouri 65409, United States.

Department of Internal Medicine, Virginia Commonwealth University Medical Center, Richmond, Virginia 23298, United States.

出版信息

ACS Pharmacol Transl Sci. 2025 Apr 9;8(5):1359-1365. doi: 10.1021/acsptsci.5c00095. eCollection 2025 May 9.

DOI:10.1021/acsptsci.5c00095
PMID:40370995
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12070320/
Abstract

Cholesteryl ester hydrolase (CEH) is a critical enzyme in cholesterol ester hydrolysis, influencing cholesterol metabolism and efflux. This study demonstrates that CEH overexpression promotes free cholesterol efflux from macrophages, thereby reducing the lipid burden in existing atherosclerotic plaques. To enable targeted delivery, galactose-functionalized polyamidoamine (PAMAM) dendrimeric nanoparticles were utilized as nanocarriers for hepatic delivery of the CEH expression vector. The therapeutic potential of CEH plasmid-loaded dendrimeric nanoparticles was evaluated in Ldlr mice. Results showed a significant reduction in total lesion area (21%) and aortic arch lesion area (23%) compared to baseline. Lesion component analysis revealed marked decreases in total cholesterol (36%), free cholesterol (35%), and cholesterol esters (44%). Collectively, these results support CEH overexpression as an effective strategy to enhance cholesterol efflux and mitigate lipid accumulation in atherosclerotic plaques. Moreover, galactose-functionalized PAMAM dendrimeric nanoparticles demonstrate strong potential as a targeted hepatic gene delivery system for therapeutic intervention in atherosclerosis.

摘要

胆固醇酯水解酶（CEH）是胆固醇酯水解中的一种关键酶，影响胆固醇代谢和流出。本研究表明，CEH的过表达促进了巨噬细胞中游离胆固醇的流出，从而减轻了现有动脉粥样硬化斑块中的脂质负担。为了实现靶向递送，半乳糖功能化的聚酰胺胺（PAMAM）树枝状纳米颗粒被用作纳米载体，用于将CEH表达载体递送至肝脏。在Ldlr小鼠中评估了负载CEH质粒的树枝状纳米颗粒的治疗潜力。结果显示，与基线相比，总病变面积显著减少（21%），主动脉弓病变面积减少（23%）。病变成分分析显示，总胆固醇（36%）、游离胆固醇（35%）和胆固醇酯（44%）显著降低。总体而言，这些结果支持CEH过表达作为增强胆固醇流出和减轻动脉粥样硬化斑块中脂质积累的有效策略。此外，半乳糖功能化的PAMAM树枝状纳米颗粒作为一种靶向肝脏基因递送系统，在动脉粥样硬化治疗干预方面显示出强大的潜力。