Chen Panyu, Wei Xihua, Que Tengcheng, Yan Tengyue, Li Shousheng, Zhong Yanli, Li Yingjiao, He Meihong, Liu Wenjian, Hu Yanling
Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi, Nanning, Guangxi, 530003, China.
Life Sciences Institute, Guangxi Medical University, Nanning, Guangxi, 530021, China.
Virol J. 2025 May 15;22(1):143. doi: 10.1186/s12985-025-02751-5.
Ticks are the second most important vectors of arboviruses after mosquitoes, and they also serve as reservoir hosts for some zoonotic diseases. It is essential to understand the prevalence of tick-borne viruses in ticks from different sampling sites and vectors, as this information can facilitate the surveillance and prevention of arboviral infectious diseases.
We systematically collected ticks from a variety of animals, including wildlife and domestic livestock, across 18 distinct regions in Guangxi Zhuang Autonomous Region(Guangxi). We then identified the ticks using traditional morphological classification and molecular biology methods to investigate the diversity of ticks in the regionWe also systematically examined the diversity of viruses carried by ticks using comprehensive virological methods based on viral metagenomics. We performed phylogenetic and recombination analyses for the assembled viral sequences.
We collected 1286 Ixodidae from 18 sampling sites in 17 districts of Guangxi. We identified 4 genera and 6 species of Ixodidae. We annotated 2 unclassified viruses and 13 known viral families. We assembled 208 nucleotide sequences and obtained six near full-length sequences of Jingmen tick virus (JMTV). Among these sequences, GXTV-PC4.2 and GXTV-43 were new mutant strains of JMTV. We detected genetic recombination of JMTV in segments 2, 3, and 4 of JMTV.
Our study uncovers a diverse tick fauna in Guangxi, including 4 genera and 6 species, and a broad virome with 13 viral families and 2 novel viruses. The JMTV, in particular, shows significant genetic diversity and potential for cross-species transmission, marked by new strains and recombination events. These findings underscore the need for vigilant tick-borne disease surveillance in Guangxi.
蜱是仅次于蚊子的第二重要虫媒病毒传播媒介，并且它们还作为一些人畜共患病的储存宿主。了解来自不同采样地点和媒介的蜱中蜱传病毒的流行情况至关重要，因为这些信息有助于虫媒病毒传染病的监测和预防。
我们在广西壮族自治区（广西）的18个不同地区系统地采集了来自各种动物（包括野生动物和家畜）的蜱。然后我们使用传统形态分类和分子生物学方法鉴定蜱，以研究该地区蜱的多样性。我们还基于病毒宏基因组学使用综合病毒学方法系统地检测了蜱携带的病毒的多样性。我们对组装的病毒序列进行了系统发育和重组分析。
我们从广西17个县的18个采样点收集了1286只硬蜱科蜱。我们鉴定出硬蜱科4属6种。我们注释了2种未分类病毒和13个已知病毒科。我们组装了208个核苷酸序列，并获得了6条荆门蜱病毒（JMTV）的近全长序列。在这些序列中，GXTV-PC4.2和GXTV-43是JMTV的新突变株。我们在JMTV的第2、3和4节段中检测到JMTV的基因重组。
我们的研究揭示了广西丰富的蜱类动物群，包括4属6种，以及具有13个病毒科和2种新病毒的广泛病毒组。特别是JMTV表现出显著的遗传多样性和跨物种传播潜力，以新菌株和重组事件为特征。这些发现强调了广西对蜱传疾病进行警惕监测的必要性。
