Shehata Ayman Ahmed, Fawzi Elshaima Mohamed, Abd El-Emam Mahran Mohamed, Abdullah Shimaa M, Hassan Wafaa, Eldin Asmaa Lbrahim Abdelaziz Zin, Elsheikh Hend E M
Department of Animal Medicine, Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
Vet Med Int. 2025 May 8;2025:9808789. doi: 10.1155/vmi/9808789. eCollection 2025.
Bovine papillomatosis virus (BPV) is a prevalent cutaneous oncogenic viral disease in cattle, causing economic losses due to reduced milk production, poor carcass quality, and hide damage. Despite BPV's economic significance, molecular information on current strains, genetic relationships, and origins in Egypt is limited, with most studies focusing on electron microscopy and histopathological analysis. The study aimed to genetically analyze BPV-1 circulation in Al-Sharkia, Egypt, and characterize viral strains compared with local and global papillomaviruses isolates. A total of 27 crossbred cattle with clinical symptoms of papillomatosis, such as wart-like lesions on various body parts, were examined. The collected tissue samples underwent histological analysis, revealing typical benign neoplasms such as hyperkeratosis and koilocytes. Polymerase chain reaction (PCR) confirmed the presence of BPV-1 in all samples, with partial amplification of the L1 gene. Sequencing and phylogenetic analysis of three representative samples indicated high similarity to BPV-1 strains from Egypt, Iraq, Turkey, and Belgium, suggesting livestock trading may play a role in disease transmission. The isolates were found to be linked to equine Delta papillomavirus 4 (DPV4) strains, indicating cross-species transmission between cattle and equines. The study marks one of the first reports of BPV-1 infection in cattle in Al-Sharkia, providing crucial molecular insights into Egypt's circulating strains and emphasizing the need for stronger biosecurity protocols in animal trading.
牛乳头瘤病病毒（BPV）是牛中一种常见的皮肤致癌性病毒疾病，由于产奶量减少、胴体质量差和皮革受损而造成经济损失。尽管BPV具有经济重要性，但关于埃及当前毒株、遗传关系和起源的分子信息有限，大多数研究集中在电子显微镜和组织病理学分析上。该研究旨在对埃及东部省BPV - 1的传播进行基因分析，并与本地和全球乳头瘤病毒分离株相比对病毒毒株进行特征描述。共检查了27头有乳头瘤病临床症状的杂交牛，如身体各部位出现疣状病变。收集的组织样本进行了组织学分析，发现了典型的良性肿瘤，如角化过度和空泡细胞。聚合酶链反应（PCR）证实所有样本中都存在BPV - 1，并对L1基因进行了部分扩增。对三个代表性样本的测序和系统发育分析表明，它们与来自埃及、伊拉克、土耳其和比利时的BPV - 1毒株高度相似，这表明牲畜交易可能在疾病传播中起作用。发现这些分离株与马的δ乳头瘤病毒4型（DPV4）毒株有关，表明牛和马之间存在跨物种传播。该研究是关于埃及东部省牛感染BPV - 1的首批报告之一，为埃及流行毒株提供了关键的分子见解，并强调了在动物交易中加强生物安全协议的必要性。
