Bosnali Wael, Korkmaz Şeyma, Mülazımoğlu Ayşen Demir, Mülazımoğlu İbrahim Ender
Institute of Science, Chemistry Department, Necmettin Erbakan University, Konya 42090, Türkiye.
Ahmet Keleşoğlu Education Faculty, Chemistry Department, Necmettin Erbakan University, Konya 42090, Türkiye.
ACS Omega. 2025 Apr 29;10(18):18270-18282. doi: 10.1021/acsomega.4c08502. eCollection 2025 May 13.
A simple, facile, and sensitive method based on leather shaving waste extract (LSWE) modified pencil graphite electrode (PGE) was developed to determine paracetamol (PAR) by employing the square wave adsorptive stripping voltammetry (SW-AdSV) technique. Leather shaving waste (LSW) was characterized by energy-dispersive X-ray spectroscopy and by investigating its morphology by taking scanning electron microscopy (SEM) images. The extraction process was conducted on an LSW by utilizing acetonitrile. Furthermore, the extraction ratio of LSW to acetonitrile was optimized and found to be 0.1 g LSW/10 mL acetonitrile at room temperature for an extraction period of 12 h. Modification of PGE by 0.1 g of LSWE (0.1LSWE/PGE) was done by performing cyclic voltammetry (CV) at the potential range 0-(+2.3) V for 10 cycles, followed by a characterization process of 0.1LSWE/PGE by employing CV, electrochemical impedance spectroscopy, and SEM techniques. PAR determination parameters at 0.1LSWE/PGE were optimized and found to be an accumulation time of 35 s in Britton Robinson buffer solution at pH 1.8. A linear relationship ( = 0.997) was observed between peak current and PAR concentration within the range 5-100 μM, with a sensitivity of 196.46 μA μM cm. The limit of detection and limit of quantification were found to be 1.6 and 4.51 μM, respectively. Neglected interferant influence on the determination of PAR at 0.1LSWE/PGE was observed in the presence of dopamine, uric acid, caffeine, ascorbic acid, Na, K, Mg, Ca, NO , and Cl ions. In order to evaluate 0.1LSWE/PGE in the determination of PAR in real pharmaceutical samples, different common PAR-containing pharmaceuticals in Türkiye were analyzed, achieving a recovery range of 99.76-102.87%.
开发了一种基于皮革削屑废料提取物（LSWE）修饰铅笔石墨电极（PGE）的简单、便捷且灵敏的方法，采用方波吸附溶出伏安法（SW-AdSV）技术测定对乙酰氨基酚（PAR）。通过能量色散X射线光谱对皮革削屑废料（LSW）进行表征，并通过拍摄扫描电子显微镜（SEM）图像研究其形态。利用乙腈对LSW进行萃取过程。此外，对LSW与乙腈的萃取比例进行了优化，发现在室温下萃取12小时时，比例为0.1 g LSW/10 mL乙腈。通过在0 - （+2.3）V的电位范围内进行10次循环伏安法（CV），用0.1 g LSWE修饰PGE（0.1LSWE/PGE），随后采用CV、电化学阻抗谱和SEM技术对0.1LSWE/PGE进行表征。优化了0.1LSWE/PGE上PAR的测定参数，发现在pH 1.8的 Britton Robinson缓冲溶液中的富集时间为35 s。在5 - 100 μM范围内，观察到峰电流与PAR浓度之间存在线性关系（ = 0.997），灵敏度为196.46 μA μM cm。检测限和定量限分别为1.6和4.51 μM。在多巴胺、尿酸、咖啡因及抗坏血酸、Na、K、Mg、Ca、NO 、Cl离子存在的情况下，观察到对0.1LSWE/PGE上PAR测定的干扰物影响可忽略不计。为了评估0.1LSWE/PGE在实际药物样品中PAR测定的性能，对土耳其不同的常见含PAR药物进行了分析，回收率范围为99.76 - 102.87%。
