Fang Chaowei, Dong Boran, Ye Shengyue, Zhang Lei, Liu Qingpo
College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou, China.
The Agricultural Industrialization Development Service Center of Tonglu County, Hangzhou, China.
Front Plant Sci. 2025 May 2;16:1556696. doi: 10.3389/fpls.2025.1556696. eCollection 2025.
Arsenic (As) is a ubiquitous and toxic metalloid in nature, posing significant risks to living organisms. Developing sustainable strategies to mitigate As stress and reduce As accumulation in rice is critical for ensuring food safety in contaminated regions. Herein, we synthesized a new nano-ferro-silicon biochar (NNFB) composed of biochar, γ-FeO, and SiO, which effectively adsorbed As from aqueous solutions and soil. NNFB alleviated As toxicity by promoting rice seeding and root growth at the seed germination and seeding stages. Under 40 μM As(III) treatment, application of 0.25% and 0.5% NNFB regulated the reactive oxygen species (ROS) balance by reducing HO accumulation and enhancing peroxidase (POD) activity in leaves. Additionally, NNFB reduced As uptake by regulating the expression of As transport genes , , and at the seeding stage. In pot experiments with 40 mg/kg As(III)-contaminated soil, NNFB application significantly improved aboveground biomass, tiller number, and effective tiller count. Notably, seed number per plant increased by 6.93- and 7.93-fold in 0.5% and 1% NNFB treatments compared to the control. These findings demonstrate that NNFB efficiently adsorbs As, mitigates As stress at multiple growth stages, and enhances rice productivity, offering a promising solution for As-contaminated agricultural systems.
砷（As）是自然界中普遍存在的有毒类金属，对生物体构成重大风险。制定可持续战略以减轻砷胁迫并减少水稻中的砷积累对于确保污染地区的食品安全至关重要。在此，我们合成了一种由生物炭、γ-FeO和SiO组成的新型纳米铁硅生物炭（NNFB），它能有效吸附水溶液和土壤中的砷。NNFB通过在种子萌发和幼苗期促进水稻幼苗和根系生长来减轻砷毒性。在40 μM As(III)处理下，施用0.25%和0.5%的NNFB通过减少叶片中HO的积累和增强过氧化物酶（POD）活性来调节活性氧（ROS）平衡。此外，NNFB在幼苗期通过调节砷转运基因、和的表达来减少砷的吸收。在含40 mg/kg As(III)污染土壤的盆栽试验中，施用NNFB显著提高了地上部生物量、分蘖数和有效分蘖数。值得注意的是，与对照相比，在0.5%和1%的NNFB处理中，单株种子数增加了6.93倍和7.93倍。这些发现表明，NNFB能有效吸附砷，在多个生长阶段减轻砷胁迫，并提高水稻产量，为受砷污染的农业系统提供了一个有前景的解决方案。
