Matsuda Y, Yamada T, Tobari I
Mutat Res. 1985 Jan-Feb;148(1-2):113-7. doi: 10.1016/0027-5107(85)90214-3.
The induction of chromosome aberrations in eggs of mice fertilized with X-irradiated sperm was performed by using an in vitro fertilization technique. Capacitated mature sperm was irradiated with various doses of X-rays and cytological analysis of the first cleavage metaphase of in vitro fertilized eggs was made. The frequencies of chromosome aberrations increased exponentially with dose and the dose-response relationship for overall breaks fitted well to a quadratic equation. The chromosome aberrations were mainly chromosome-type (82.1%), and the majority of aberrations were fragments.
利用体外受精技术，对用X射线照射过的精子使小鼠卵子受精后诱导染色体畸变进行了研究。对获能的成熟精子用不同剂量的X射线进行照射，并对体外受精卵第一次卵裂中期进行细胞学分析。染色体畸变频率随剂量呈指数增加，总断裂的剂量-反应关系很好地符合二次方程。染色体畸变主要为染色体型（82.1%），且大多数畸变是片段。
