Lei Zheng, Zhang Hua, Liu Wenju, Sheng Jiandong, Zhang Huan, Wang Yin, Tang Yanni, Wang Huaxing, Ding Cuicui, Qiao Wanqi, Zhu Yonghui, Yang Guoyin, Zhang Yihan, Liu Zhuoyi, Zhou Nanyu, Hu Chengxiao, Zhao Xiaohu
College of Resources and Environment, Huazhong Agricultural University, Research Center of Trace Elements, Wuhan, Hubei, 430070, China.
State Key Laboratory of Environmental Geochemistry, Guiyang, Guizhou, 550081, China.
Adv Sci (Weinh). 2025 Jul;12(25):e00862. doi: 10.1002/advs.202500862. Epub 2025 May 19.
Rhizosphere microbiome is crucial for regulating rhizosphere complex nutrient dynamics. However, mechanisms by which plants regulate rhizosphere microbes to manage nutrient availability under coexisting beneficial and harmful elements remain unclear. This study focuses on the rhizosphere microbiome of Brassica napus in different naturally selenium (Se)-cadmium (Cd)-rich soils, the functionality of this rhizosphere, and the changes in the availability of rhizosphere nutrients. Microbiome analysis, metagenomics, genomic analysis, strain isolation, and functional validation are performed to investigate these relationships. Results show that a significant negative correlation is observed between the rhizosphere available Se and Cd content across the plant whole growth cycle and identified a group of core microbiota that are highly positively correlated with available Se and negatively correlated with available Cd. Genomics and metagenomics analyses reveal that the core microbiota has a higher substrate preference for amino acids related to the glutathione metabolic pathway. Key glutathione-related-amino acids and synthetic microbial community significantly improve the expression of glutathione anabolism and related amino acid transport genes and enhance Se uptake and reduce Cd absorption in plants grown in various Se-Cd-rich soils. This study provides insights into the mechanisms of root-associated microbes responding to complex soil nutrients during plant growth.
根际微生物群对于调节根际复杂的养分动态至关重要。然而，在有益元素和有害元素共存的情况下，植物调节根际微生物以控制养分有效性的机制仍不清楚。本研究聚焦于不同自然富硒镉土壤中甘蓝型油菜的根际微生物群、该根际的功能以及根际养分有效性的变化。通过进行微生物群分析、宏基因组学、基因组分析、菌株分离和功能验证来研究这些关系。结果表明，在植物整个生长周期中，根际有效硒与镉含量之间存在显著负相关，并鉴定出一组核心微生物群，它们与有效硒高度正相关，与有效镉负相关。基因组学和宏基因组学分析表明，核心微生物群对与谷胱甘肽代谢途径相关的氨基酸具有更高的底物偏好性。关键的谷胱甘肽相关氨基酸和合成微生物群落显著提高了谷胱甘肽合成代谢和相关氨基酸转运基因的表达，并增强了在各种富硒镉土壤中生长的植物对硒的吸收，减少了对镉的吸收。本研究为植物生长过程中根际相关微生物响应复杂土壤养分的机制提供了见解。
