Center for Plant Water-use and Nutrition Regulation and College of JunCao Science and Ecology, Joint International Research Laboratory of Water and Nutrient in Crop, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Center for Plant Water-use and Nutrition Regulation and College of JunCao Science and Ecology, Joint International Research Laboratory of Water and Nutrient in Crop, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plant Commun. 2024 Aug 12;5(8):100930. doi: 10.1016/j.xplc.2024.100930. Epub 2024 Apr 29.
Plants can shape their root microbiome to promote growth and nutrient uptake. PHOSPHATE STARVATION RESPONSE 2 (OsPHR2) is a central regulator of phosphate signaling in rice, but whether OsPHR2 can shape the root microbiome to promote phosphorus uptake is unclear. Here, we investigate the role of OsPHR2 in recruiting microbiota for phosphorus uptake using high-throughput sequencing and metabolite analysis. OsPHR2-overexpressing (OsPHR2 OE) rice showed 69.8% greater shoot P uptake in natural soil compared with sterilized soil under high-phosphorus (HP) conditions, but there was only a 54.8% increase in the wild-type (WT). The abundance of the family Pseudomonadaceae was significantly enriched in OsPHR2 OE roots relative to those of WT rice. Compared with the WT, OsPHR2 OE rice had a relatively higher abundance of succinic acid and methylmalonic acid, which could stimulate the growth of Pseudomonas sp. (P6). After inoculation with P6, phosphorus uptake in WT and OsPHR2 OE rice was higher than that in uninoculated rice under low-phosphorus (LP) conditions. Taken together, our results suggest that OsPHR2 can increase phosphorus use in rice through root exudate-mediated recruitment of Pseudomonas. This finding reveals a cooperative contribution of the OsPHR2-modulated root microbiome, which is important for improving phosphorus use in agriculture.
植物可以塑造根际微生物组以促进生长和养分吸收。PHOSPHATE STARVATION RESPONSE 2 (OsPHR2) 是水稻中磷酸盐信号的核心调节剂，但 OsPHR2 是否可以塑造根际微生物组以促进磷吸收尚不清楚。在这里，我们使用高通量测序和代谢物分析研究了 OsPHR2 在招募微生物群以吸收磷中的作用。与灭菌土壤相比，在高磷 (HP) 条件下，OsPHR2 过表达 (OsPHR2 OE) 水稻在自然土壤中的地上部 P 吸收量增加了 69.8%，而野生型 (WT) 仅增加了 54.8%。与 WT 相比，OsPHR2 OE 根中的假单胞菌科的丰度明显富集。与 WT 相比，OsPHR2 OE 水稻中琥珀酸和甲基丙二酸的丰度相对较高，这可能刺激了假单胞菌 (P6) 的生长。在低磷 (LP) 条件下，接种 P6 后，WT 和 OsPHR2 OE 水稻的磷吸收量均高于未接种的水稻。总之，我们的结果表明，OsPHR2 可以通过根分泌物介导的假单胞菌的招募来增加水稻中的磷利用。这一发现揭示了 OsPHR2 调节的根际微生物组的协同贡献，这对于提高农业中的磷利用至关重要。
